FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497762
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC762233.0516518387260274No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN478011.9137531918573507No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT407371.6309400175036692No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA389861.5608372615165096No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA367141.4698758328455634No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN293861.1764931967096945No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT283221.1338950628602726No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA244890.9804376878181347No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA223540.8949611692387024No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA183380.7341772354611849No Hit
CGCCTTGGCCGTACAGCAGCCTTTAACGAAGAGAA177280.7097553730099184No Hit
CTGACCTATGAATTGACAGCCCGCCTTCGCCGTAC177090.7089946920483217No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN171470.6864945499210894No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT149550.5987359884568666No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147700.5913293580413186No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA136510.5465292529872742No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG136090.5448477477037443No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC121260.4854745968591083No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA117330.4697405117060792No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTCGCCGT111250.44539872093498095No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC103350.4137704072685868No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT100540.4025203362049707No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTCGCCGT94340.3776981153528639No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGCCGTA92940.37209309774109783No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA88810.355558295786388No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT82360.32973517893218013No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT82070.32857413956974285No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA81670.32697270596638106No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA81080.32461059140142257No Hit
TAGCACCATCTGAAATCGGTTACGCCTTCGCCGTA80910.3239299821199938No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT78330.3136007353783107No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC77720.311158549133184No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC66110.26467693879560983No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTCGCCGT65540.2623948959108194No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA62350.24962346292400958No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT60680.24293747762997434No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA60480.2421367608282935No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTCGCCGTA59420.2378929617793849No Hit
TGACCTATGAATTGACAGCCAGCGCCTTCGCCGTA53190.21295063340702594No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC52750.21118905644332808No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG52370.20966769452013442No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTCGCCCA51270.20526375211088968No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC50210.2010199530619811No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG49530.19829751593626616No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA49500.19817740841601403No Hit
CGCCTTCGCCGTACAGCAGCCTTTAACGAAGAGAA49450.19797722921559383No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA48110.192612426644332No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC47310.18940955943760854No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTCGCCGTA46390.1857262621498766No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA46220.18504565286844782No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA42330.169471711075755No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTCGCCGT38280.1532571958417175No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA37410.14977407775440574No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT37390.14969400607423766No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA36270.14520999198482482No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT36210.14496977694432056No Hit
ACCCTGTAGATCCGAATTTGTGTCGCCTTGGCCGT35830.14344841502112693No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA35610.14256762653927796No Hit
TCCCCGGCATCTCCACCACGCCTTCGCCGTACAGC35390.14168683805742902No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA34480.1380435766097811No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC33560.1343602793220491No Hit
TACCCTGTAGATCCGAATTTGTGTCGCCTTGGCCG33220.13299906075919163No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT33020.13219834395751076No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTCGCCG32490.1300764444330565No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32390.12967608603221603No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC31750.12711379226683728No Hit
ATCACATTGCCAGGGATTTCCACGCCTTCGCCGTA31580.12643318298540854No Hit
TAGCTTATCAGACTGATGTTGACGCGGAAGCGGCA31490.12607286042465216No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC29900.11970716185128927No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA29490.11806569240784351No Hit
CTGACCTATGAATTGACAGCCGCCTTCGCCGTACA29360.11754522648675095No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA29350.1175051906466669No Hit
TCACTCCTCTCCTCCCGTCTTACGCCTTGGCCGTA29330.11742511896649879No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTCGCCAC28890.11566354200280092No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT28870.11558347032263282No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT28520.1141822159196913No Hit
TAGCTTATCAGACTGATGTTCTGCGCCTGTTAACA28500.11410214423952322No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA28300.11330142743784236No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTCGCCGTA28100.1125007106361615No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT27050.10829694742733695No Hit
TAGCTTATCAGACTGATGTTGACATAAGGATAATG26980.10801669654674866No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA25960.10393304085817624No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC25820.10337253909699963No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA25780.10321239573666345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)