FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4106232
Sequences flagged as poor quality0
Sequence length35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2035574.957269827910356No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1528303.721903682013096No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN855772.084076106756754No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA823702.0059753077760827No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT491231.1963035697934261No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC464531.1312804537103602No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN452921.1030063571663755No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT418951.0202784450561975No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT416081.013289068907943No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA310710.7566791160363078No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA307760.7494949140720739No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277050.6747061539630493No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA263560.6418536507435527No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT229700.5593936241303462No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN225260.5485807913434994No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT221040.5383037295505954No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA200130.48738113189902565No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA192010.4676063115771345No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA186630.45450427545253164No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT185700.4522394253417732No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT184940.45038858008997057No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA171930.4187050317663493No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA161480.3932559095540632No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA160950.3919651885232008No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA142700.3475205492529404No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA138120.33636677128812986No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC134520.3275996095690648No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT120790.2941626289016305No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA117930.28719760598037325No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGT116150.28286273157483555No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC114740.2794289265682017No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA109990.2678611437444353No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA109960.26778808406344307No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA106180.25858256425842474No Hit
TAACACTGTCTGGTAACGATGTTCGCCTTGGCCGT103410.25183672038014415No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA102960.25074082516526097No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA99900.2432887377040557No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT99790.24302085220708425No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA97110.23649418737178027No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA94080.22911515959156717No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT90590.22061588336947355No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA85110.2072703149748967No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGG85010.20702678270492267No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA84290.20527335036110966No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC83540.20344685833630444No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA83200.20261884861839272No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC81920.19950163556272515No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT80750.196652308004029No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG80010.1948501692062212No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG76300.18581512199018466No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT74000.180213879780782No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC71610.17439345852840268No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC71370.17380898108046502No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA71330.1737115681724754No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA71260.17354109558349357No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG71170.17332191654051693No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA70980.1728592052275663No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65970.16065823850186742No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA62880.1531330913596699No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAA61500.14977234603402828No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT61460.14967493312603866No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA60290.1468256055673425No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG60020.14616806843841262No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC60020.14616806843841262No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA59150.14404933768963857No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT57460.13993364232707747No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGAC56890.13854550838822552No Hit
TTCAAGTAATCCAGCTATCGCTCGCCTTGGCCGTA56640.13793667771329043No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC55640.13550135501355012No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG55210.13445416625266182No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT55060.13408886784770077No Hit
TAATACTGTCTGGTAAAACCGTCGCCTTGGCCGTA54310.13226237582289552No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA53830.1310934209270202No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT53800.131020361246028No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA52770.12851197886529547No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT51910.12641760134351882No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA51300.12493205449667724No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC50710.12349521410383046No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT49270.11998834941620445No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC48090.1171146686305109No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC48050.11701725572252128No Hit
TGTAACAGCAACTCCATGTGGAAACGCCTTGGCCG47730.1162379524586044No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC47230.11502029110873424No Hit
TGTAACAGCAACTCCATGTGGACGCCTTGGGGCAT46730.11380262975886409No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA44430.1082013875494614No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA44260.10778738269050556No Hit
ACAGTAGTCTGCACATTGGTTACGCCTTGGCCGTA42040.10238096629708208No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC41750.10167472271415741No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG41530.10113895172021453No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA41510.10109024526621974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)