FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988596
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA785343.949218443565209No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC536692.6988387787162402No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT399402.0084521944125404No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN294601.4814472120028404No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA291321.4649531629350556No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA229491.154030280660325No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA212911.0706548740920732No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA200021.0058352727250783No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT184750.9290474284369474No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT180300.9066698313785202No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN171200.8609089025624109No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC170960.8597020209233046No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA162060.8149468268064505No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT121180.6093746542786971No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT109210.5491814325282762No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101570.5107623670167294No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86610.4355334115124439No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT85150.42819154820788136No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG83560.4201959573488029No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA82060.41265294710438927No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT80270.4036516215460556No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC78530.3949017296625358No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT72780.3659868570589501No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC68430.34411212735015057No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA66110.33244560483879076No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGAT66070.33224445789893975No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA64530.32450030071467506No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA60640.3049387608141624No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA57990.2916127760490316No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT57740.29035560767496266No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA54260.27285582390792296No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC53880.27094492797933817No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA52910.2660671146879507No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA49820.2505285135844586No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC48350.24313636354493323No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA47040.23654880126481198No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT45240.22749718897151558No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA44140.22196564812561226No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA43910.22080905322146882No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA42230.21236088174772555No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA40600.204164143948796No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA40290.20260525516495057No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC39620.1992360439224458No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT37470.1884243959054529No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA37250.18731808773627223No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC34500.1734892356215139No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT33080.16634851925680227No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACC31980.16081697841089895No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA31510.15845350186764934No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA30910.15543629776988388No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG30860.1551848640950701No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT30130.15151393244278877No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT29670.14920074263450192No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT28830.14497665689763028No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC28060.14110457830549794No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT27900.14029999054609382No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC27230.13693077930358905No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACC27150.13652848542388699No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA25950.13049407722835607No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG25810.1297900629388775No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC24360.12249848636927763No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA23160.11646407817374671No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT22950.1154080567395288No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA22320.11223999243687506No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA22190.1115862648823592No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA22020.11073139038799233No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA21950.11037938324325303No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC21780.10952450874888615No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA21380.10751303935037586No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC21070.10595415056653035No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC20780.10449583525261039No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG20140.10127748421499388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)