FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061343
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2027326.6223222944962385No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1064853.478375340495985No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT656242.143634346102348No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT499981.6332047731992136No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA489241.5981221313652212No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA471301.53952040003358No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA433891.4173191308520476No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT368951.2051900097440895No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA300510.9816279979081077No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC242660.7926586468749173No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA235260.7684862493356673No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA170460.5568144438568301No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT167030.545610210943367No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC158270.516995318721228No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA140520.45901423002910813No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA139140.4545064045420588No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA114170.37294089554813037No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT108290.3537336391250507No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG108100.3531129964855294No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC104570.34158210955126556No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT94450.3085247226462373No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT92890.30342891992174675No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT87560.2860182606130708No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT80720.26367512559030465No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC80310.26233584410502186No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC78610.2567827257514104No Hit
CGCCTTGGCCGTACAGCAGATACCGACAACTCAAC76900.25119694199571885No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA73110.2388167546073733No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68510.22379066965054228No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA67910.22183074552573823No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC67220.21957683278221357No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA65500.21395838362444197No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT64220.20977721215819334No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA59220.19344451111815958No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA57530.18792405816662816No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC57330.1872707501250268No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA56810.18557214921686332No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA56530.18465751795862143No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC56050.18308957865877817No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA54120.17678515605732517No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA53100.17345328504515828No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC52750.1723099959723559No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA52260.1707093912704326No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG50800.16594024256674278No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA50490.16492761510226067No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT50170.1638823222356985No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC46770.15277608552847557No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT45980.15019551876415024No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGACCA43960.14359710754397662No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA42910.14016724032556951No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA42870.14003657871724925No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA42780.13974259009852866No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC41530.1356594148385202No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40930.13369949071371617No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC39080.1276563913289037No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT38820.12680709087482192No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG38820.12680709087482192No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA38480.12569646720409963No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC38480.12569646720409963No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38000.1241285279042564No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA37740.12327922745017465No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT36240.11837941713816452No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA36090.11788943610696351No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC35940.11739945507576251No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT35830.11704013565288175No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC34870.11390425705319529No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA34630.11312028740327366No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC33670.10998440880358717No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC32860.10733851123510173No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT30900.10093609242740849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)