FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161222
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1993424.790467800083725No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1509403.6272998652799586No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1146312.754743678659778No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA622191.495209820576744No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC488131.1730448411548338No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA478371.1495901924963388No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT451761.0856426309387002No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT366850.8815919938902563No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT343080.8244693505898028No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA304260.7311794468067313No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA295770.7107767862421183No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA295340.7097434359426149No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN245530.5900430210164226No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA230870.554812985224052No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGCA224940.5405623636518311No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN197090.47363490820725257No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA178740.42953728496100424No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC162880.39142348089094986No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA162440.3903660991891324No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC162330.39010175376367806No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN161980.38926065468268695No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA156430.3759232263983993No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT152680.3669114505306374No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA143400.3446103091832159No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT141650.3404048137782603No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATAC141280.33951565189264116No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGAC133740.3213959745478612No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC132440.31827189224703706No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA130270.3130570779448922No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA124540.29928708441895197No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA121010.29080399940209867No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114990.2763370952090516No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA105650.2538917654477459No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA99290.23860779357602166No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC95770.23014873996148247No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT91140.21902220069008574No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA91040.21878188666694542No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG86680.20830419525802757No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA86150.20703053093538387No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA84120.20215215626563543No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT82420.19806681787225006No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA80650.1938132596626664No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80500.19345278862795592No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77380.185954991105978No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC76550.18396038471391335No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC76360.18350378806994677No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC74700.1795145752858175No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC73130.17574164512251447No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC71860.17268965702863245No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71810.1725695000170623No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC70100.16846013022136286No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC67390.16194762019426026No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA67060.1611545839178972No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG64870.15589170681112424No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA64640.15533898455790152No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC63570.1527676245103001No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA62510.15022029586501273No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC61230.14714427636881666No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATCA61210.1470962135641886No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT60420.14519773278138007No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT60330.1449814501605538No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA60280.14486129314898363No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA59510.1430108751708032No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCTAC59050.14190543066435773No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC55030.13224480693411694No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA54680.13140370785312583No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA54320.1305385773698207No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC54290.1304664831628786No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC53780.12924088164486297No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC53690.12902459902403668No Hit
TGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52600.12640517617180722No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC52500.1261648621486669No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA52430.12599664233246868No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT50990.1225361203992481No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC49620.11924381828222574No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG49310.11849884481049076No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC48560.11669648963693838No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA48550.11667245823462435No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA46850.11258711984123895No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA46160.11092895308157075No Hit
AGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46080.11073670186305849No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC45150.10850178144785354No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA43710.10504125951463297No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA42930.10316681013413849No Hit
CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42320.10170089459298254No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC42190.10138848636290013No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA41880.10064351289116515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)