FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239027
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT441621.3634341424137557No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA407791.2589891964469575No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN312750.9655677461163491No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT312640.9652281379562443No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT235280.7263909809952186No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA227720.7030506383552838No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC224230.6922757976392293No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC155840.4811321424613009No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT155430.4798663302281827No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC151720.46841227319191847No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145190.4482518978693293No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA140560.4339574816758242No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA115450.3564342007646123No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA115370.3561872130118088No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT114060.35214278855965075No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA109080.33676780094763026No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC103620.3199108868187885No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT100630.3106797195577561No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC98700.30472114002137063No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTA87760.27094556482548615No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC86440.2668702669042277No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79890.24664814464343768No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA77750.2400412222559429No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT76260.23544107535997694No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76200.23525583454537427No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA73030.22546894484053392No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC72060.22247421833779096No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA70850.21873852857663736No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA69900.21580554901209528No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACCG69570.2147867245317807No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC62950.19434848798728754No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC60620.18715496968688436No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA56460.17431160654110014No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55420.1711007657546541No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA55360.17091552494005144No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT55100.17011281474343992No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG53570.16538917397107217No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT50530.15600363936453754No Hit
TGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47870.14779129658381976No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC44460.13726344362056878No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA44310.13680034158406212No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC43730.13500968037623645No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTG43410.13402172936502227No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCTAC42270.13050215388757178No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC42230.13037866001117002No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA42100.12997730491286427No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG42040.1297920640982616No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC41920.12942158246905627No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA41420.12787790901403415No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA41160.12707519881742263No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40960.12645772943541378No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC40600.12534628454779784No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA40210.12414221925288056No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA40190.12408047231467968No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA40150.12395697843827791No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT40110.12383348456187615No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACCA39160.12090050499733408No Hit
TGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38470.11877023562940352No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA38180.11787490502549068No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG37360.11534328055925437No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36830.1137069866969309No Hit
AGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36490.11265728874751584No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA36080.1113914765143977No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC34440.10632822758192507No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC34330.1059886194218202No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA34210.10561813779261488No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA33170.10240729700616884No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA32840.1013884725258542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)