FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879177
Sequences flagged as poor quality0
Sequence length35
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT55103119.13848992264109No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA32009111.117447798450737No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC843632.9301081524338377No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA554571.926140699234538No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN412991.4344029561225309No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC391501.3597635713261116No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT259560.9015076183228748No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG236790.8224225186572414No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT231640.8045354627381367No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT231140.8027988553673497No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN219360.7618843857116113No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA216190.7508742949808227No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA206080.7157600939435124No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA194280.6747761599929425No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA175870.6108342766005702No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA174190.6049992758347265No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA173510.6026374898104562No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA165490.574782307583035No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA137830.47871318783110595No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG137760.47847006279919574No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124480.4323457710310967No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111590.3875760330122115No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG105110.3650696014868138No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC96020.33349807948590865No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG93810.32582227490703075No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA83910.2914374489654509No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA83550.29018709165848433No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA81620.2834837872072471No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT78430.2724042321816269No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG77550.269347803209042No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT75900.2636169988854454No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA73200.25423931908319636No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT72260.25097449722611703No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC70830.24600780014566664No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA62650.21759690355959357No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA55120.19144359655554347No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC53890.18717154242340778No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT53710.18654636376992453No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC50770.1763351124296978No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC49900.17331341560452865No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA49280.17116002246475295No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC49030.1702917187793595No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGAGAT48710.16918029006205593No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA47440.1647693073402573No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC47240.16407466439194257No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC46500.161504485483178No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC44710.15528743109576104No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA44610.15494010962160368No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT44320.1539328773465473No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT43520.15115430555328832No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC43340.15052912689980505No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA41430.14389528674339924No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA41190.14306171520542155No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA41040.1425407329941855No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT40250.13979689334834225No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA39160.1360110892800269No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA37160.12906465979687945No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA36230.1258345700872159No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA35520.12336858762069855No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC33160.11517180083058458No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC32630.11333099701755049No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC31380.10898947859058336No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA31070.10791278202069549No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT30690.10659296041889749No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT30230.10499528163777357No Hit
GTCCAGTTTTCCCATTCCGAAATAAGGTTAATGCA30160.1047521566058634No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC30070.10443956727912178No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA30020.10426590654204308No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA29810.1035365314463126No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA29810.1035365314463126No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA29170.10131367401170542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)