FastQCFastQC Report
Wed 8 May 2024
EGAF00002406322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4040088
Total Bases141.4 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1044712.5858595159313364No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA990582.4518772858412983No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN626481.5506592925698648No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT591801.4648195781874058No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA564771.3979150949187245No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT554171.3716780426564965No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA510051.262472500598997No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT440131.0894069634126782No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT396730.9819835607541221No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA386410.9564395627025947No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA334750.828571060828378No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297840.7372116646964125No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT284690.7046628687295919No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA259030.6411494007061233No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA236630.5857050638500944No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA229290.5675371427553063No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA226590.5608541200092672No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN225840.558997724802034No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA219940.5443940825051335No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC185470.4590741587806998No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC182730.4522921282902749No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAC147340.36469502644496854No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA145890.3611059957109845No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA144570.3578387401462543No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA144190.3568981665745895No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA141900.35122997320850435No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC140240.34712115181649505No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC137980.3415272142586993No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC136120.3369233541447612No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133890.33140367239525476No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC126680.31355752646972046No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA125730.31120609254055853No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC122410.30298844975653993No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCACA116880.2893006290952078No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA113240.28029092435610314No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA112030.2772959400884337No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA109600.27128121961699847No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA108420.26836049115761834No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAA105830.26194973970864No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT103580.25638055408694066No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT93870.232346424137296No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA92390.22868313759502268No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT91110.22551488977467815No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC88300.21855959573157813No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC83620.20697568963844354No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC78910.1953175277370196No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA78340.19390666737952242No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC77200.1910849466645281No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTG76260.1887582646714626No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT75770.18754541980273695No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA74900.18539200136234657No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT74030.18323858292195613No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT68480.16950125838843114No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA66620.16489739827449304No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA65830.16294199532287415No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA64320.15920445297231148No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTT62810.15546691062174883No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG62140.15380853090328728No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC61920.15326398830916554No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC61660.15262043797065805No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC60110.14878388787570965No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT60100.14875913593961318No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG57840.14316519838181743No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT53270.13185356358574368No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG53120.13148228454429706No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG52940.1310367496945611No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT49650.12289336271883186No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA49040.12138349461694893No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG48050.11893305294340124No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC47890.11853702196585818No Hit
TGGCTCAGTTCAGCAGGATGTCAGCGGAACCGGCA47320.11712616160836099No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA46850.11596282061182826No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCATG46360.11474997574310263No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA45780.11331436344950903No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG45540.11272031698319443No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT45000.11138371243398658No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT44670.11056689854280402No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA44590.11036888305403249No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC44180.10935405367407838No Hit
AACTGGCCCTCAAAGTCCCGCTCGCCTTGGCCGTA43980.10885901495214956No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCAGT43880.10861149559118514No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA43500.10767092201952036No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43420.10747290653074883No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA43160.10682935619224136No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC42010.10398288354115058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)