FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3202846
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC40140112.532635037713332No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA2019856.306422475510843No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT631281.9709970445035445No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA624031.9483609265009934No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC528821.6510940582219686No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA439241.3714053064056155No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC345381.0783534394098249No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA339601.0603069894712391No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA288860.9018853856851062No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA285130.8902394932506903No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT279590.8729423768735681No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA199050.6214785225390169No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198560.6199486331843617No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA189980.5931599583620318No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC186080.5809832879882454No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT170190.5313711617729981No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT145680.4548454718085103No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT135700.42368568454430844No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA135600.4233734622270318No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG125430.3916204525600045No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC122610.38281578321280507No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA122290.3818166717975201No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA115650.3610851099303557No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT108660.33926076995272325No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC106700.33314121253410245No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT87930.2745370835812899No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT86150.26897952633376687No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC81570.2546797442024999No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA81380.2540865217996744No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA81180.25346207716512126No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT77830.2430026295363561No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC70950.22152173410772796No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA68940.21524606553046882No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC68250.2130917315412605No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT68000.21231117574806907No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT67510.21078128639341387No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT64180.200384283228104No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG58070.18130749964250545No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55860.1744073864306932No Hit
CTGACCTATGAATTGACAGCCCATTCCAATTACGT55840.17434494196723788No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA51930.1621370493617239No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA49470.15445638035672024No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT47180.14730648929108675No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA47160.14724404482763143No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC46850.14627615564407404No Hit
CTGACCTATGAATTGACGATTTATTCCAATTACGT45720.14274804345884878No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT43090.13453659651447494No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA43000.13425559642892604No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC42350.1322261513666283No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC42200.13175781789071345No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC41460.1294473727428668No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40690.1270432608998372No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT40530.12654370519219468No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA40110.1252323714596331No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC39360.1228907040800588No Hit
CTGACCTATGAATTGACAGCCCGCCTTAATTACGT38630.12061148116393981No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG38300.11958114751692712No Hit
CTGACCTATGAATTGACGATTATTCCATAATGTGT37910.1183634804795485No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG35190.10987103344962575No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34970.10918414435161729No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT34710.1083723663266982No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT34580.10796647731423864No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG32970.10293969800608584No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT32330.10094147517551579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)