FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547258
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1178523.3223408052078534No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA856252.4138362645175513No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT291730.8224098726396558No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA286060.8064256955654199No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA274360.7734424730312822No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA271150.7643932299257624No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA206250.5814350126210159No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC200010.5638439606028093No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT175520.494804719589046No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT172290.4856990949065447No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA162660.45855136558998527No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG161130.4542381749509057No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT156050.4399172543976221No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA132850.3745146250991611No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA131270.370060480517628No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA128940.36349202679929116No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA119370.336513442213676No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC109350.3082662721459787No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC103220.29098531880116985No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC99030.2791733784235598No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT98140.27666439824788613No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA95380.2688837406244485No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88120.24841722818018877No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC83420.23516755758955227No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC78610.2216077883255179No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGAT76480.21560315037699543No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT67730.190936210447619No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGT66150.18648206586608587No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66010.18608739482721584No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA64210.18101305289888697No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT64190.18095667132190552No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC62550.17633338200942814No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT62410.1759387109705581No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC60460.1704415072148685No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA60130.16951121119467485No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA57790.16291456668784735No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC57080.16091302070500652No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACCTA56040.15798117870197206No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGT55730.15710726425875984No Hit
TCGATTCCCCCACGGGCTACCACGCCTTGGCCGTA54560.15380894200534612No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA54420.15341427096647609No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT53800.1516664420800517No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGTG53780.15161006050307027No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACCTA52780.14879098165399865No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC51130.14413950155303054No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG49980.14089756087659822No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACCT49620.13988269249093244No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGT49510.13957259381753456No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC46740.13176374540560626No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC45570.12846542315219248No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGTG44970.12677397584274952No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA44840.12640749559237022No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACCTA43810.12350384437782647No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA43600.12291183781952145No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA43220.12184058785687424No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAT43070.12141772602951349No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACCTG41770.11775292352572042No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG40790.11499022625363027No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA40770.11493384467664884No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40510.11420088417589024No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39630.11172009478870723No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA39610.11166371321172579No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACCTG39280.11073341719153215No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC39270.11070522640304145No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT38210.10771700282302554No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC37490.10568726605169401No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGTG37460.10560269368622187No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA37410.1054617397437683No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT37270.10506706870489826No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA36310.10236075300978954No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36220.10210703591337308No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA35820.10097940437374445No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35600.1003592070269487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)