FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136118
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1640055.229554500181434No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1156943.689083127611908No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1099133.504746951485881No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA519481.6564427741558192No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA452801.4438232234884019No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT413771.319369998195221No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT338151.0782438670993886No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA311380.9928835585905886No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC278110.8867969891439034No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA216350.6898656236787009No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA204320.6515060976659679No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190220.6065460547084006No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC178030.5676763438110428No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA171810.5478429064212508No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140700.4486438329170012No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA136190.4342629964816375No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT129500.4129308909932598No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATCCA127360.4061071681614021No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA124230.3961266763559279No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN118820.37887604994454926No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113930.36328352440820144No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT97530.3109895737341516No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC94040.29986116593827145No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT92610.2953013885319366No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC91540.29188952711600774No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA89060.2839816614043221No Hit
CGCCTTGGCCGTACAGCAGATACCGACAACTCAAC83440.26606141733187333No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC83430.26602953077658426No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA82360.2626176693606554No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA75580.2409985848746763No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT72540.23130507206680362No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA69900.22288702147049314No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA65160.20777279426348114No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC63490.202447739530209No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT60970.1944123275973672No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC60410.19262668050118012No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60010.19135121828961793No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA59650.19020330229921195No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT58960.18800312998426716No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA58080.18519711311883036No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCTAC57440.18315637358033085No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG55540.17709792807541042No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGACCA53960.17205985233973978No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT53510.1706249573517323No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53210.16966836069306065No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA50790.1619518143131094No Hit
TGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50710.16169672187079695No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT50110.15978352855345368No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT49740.15860372600775863No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC48860.1557977091423218No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG48780.15554261670000938No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC47500.15146113762301036No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT47020.14993058296913572No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC45340.14457364168057452No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATCGT45010.1435213853560357No Hit
AGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44160.14081102815646604No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC43690.13931236005788047No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC43390.13835576339920883No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA41640.13277561622362424No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA41270.13159581367792922No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA40920.13047978424281229No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC40770.1300014859134765No Hit
TAGCTTATCAGACTGATGTTGACATAACACCGAAC40430.1289173430336486No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC40210.1282158388172894No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39700.1265896244975476No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC38810.12375172107682171No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT38460.1226356916417048No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT38260.12199796053592371No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATTTG37980.12110513698783017No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC36870.11756572935074508No Hit
CAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36690.1169917713555421No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC36210.11546121670166748No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36110.11514235114877691No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA35180.11217690150689484No Hit
ACTGGACTTGGAGTCAGAAGGCACGCCTTGGCCGT35130.11201746873044954No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC34860.11115653173764507No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA34780.11090143929533264No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC34760.11083766618475452No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA32970.10512997278801371No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC32750.10442846857165451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)