FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025234
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA596531.4819759546898392No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA545631.355523678871837No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT496231.2327978944826563No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT350440.8706077708774198No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA317520.7888237056528887No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310670.7718060614612715No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA281430.6991643218754487No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA277700.6898977798557798No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAGCCGT270970.6731782549784684No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT268190.6662718241970529No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA246130.6114675569171879No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATTAC244100.6064243718501832No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT226230.5620294372948256No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCGT187600.46605986136458155No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT176020.4372913475340813No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTCGGCA163770.4068583341987075No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAGCTAC159540.3963496283694314No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC155150.3854434301210811No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC155130.3853937435686969No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTTAATG142420.35381793952848456No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTCGATG140980.35024050775681614No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG134050.33302411735566184No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT133350.33128508802221185No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGATTAC127960.3178945621546474No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC122230.30365936489655015No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA117220.29121288352428704No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA116750.2900452495432564No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG95710.23777499643498987No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC94800.23551425830150496No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT92830.230620132891653No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA92710.2303220135773473No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN92000.22855814096770524No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA88020.21867051704323276No Hit
GCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86650.21526698820490933No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC86000.2136521752524201No Hit
TGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85650.21278266058569517No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA82360.20460922271848045No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGATCGT77590.1927589799748288No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT77550.19265960687006023No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT74230.18441163917426912No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA74200.1843371093456927No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAATCGT73780.18329369174562274No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA73520.18264776656462706No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA72300.17961688686918575No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71850.17849893944053935No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC71810.1783995663357708No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71480.17757973822143008No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC71430.1774555218404694No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA68740.17077268054478323No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC67800.1684374125827219No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC65540.16282283216329782No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62950.1563884236295331No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTTAGCA61520.1528358351340568No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCTAC60040.1491590302576198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG58500.14533316572402996No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58230.14466239726684213No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC57540.14294821120958434No Hit
GACTCTTAGCGGTGGATCGCCTTGGCCGTACAGCA56730.14093590583802085No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA56490.14033966720940944No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA55940.13897328701884165No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA54510.13542069852336536No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA53900.13390525867564468No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA53330.13248919193269262No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA52950.1315451474373912No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG52110.1294583122372513No Hit
CAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51900.12893660343721633No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC51620.12824099170383635No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51440.1277938127323778No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC50310.12498652252266576No Hit
CCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50080.1244151271702465No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT50030.1242909107892858No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTCGGCA48460.12039051642711951No Hit
AGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47550.1181297782936346No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTCGTAC46010.11430391376004476No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT44390.11027930301691778No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT43910.10908682575969497No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG43640.10841605730250714No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAGCATG42840.10642859520713578No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC42490.10555908054041081No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA42210.10486346880703085No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT41900.10409332724507445No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACTAATG41560.10324865585454163No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT41260.10250335756877738No Hit
GCCGCCGGTGAAATACCACTACTCCGCCTTGGCCG40850.10148478324489955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)