FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412916
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2380005.393259241734944No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA727971.6496348446242801No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN708411.605310411528341No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT623741.413441814890653No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN482601.0936079454039007No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA481901.0920216926857433No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN478951.0853367705163661No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG433150.9815505212426431No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC369700.8377680427182389No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA336890.7634181117428929No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA335840.7610387326656569No Hit
GTCCAGTTTTCCCAGGAATCCCCCGCCTTGGCCGT312260.7076046768168711No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC276450.6264565199065651No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA270310.6125428174930136No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246460.5584969213100816No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT214110.4851893849780961No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA211660.47963750046454545No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC206640.46826180240004567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC205800.46635829913825694No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA187420.4247078349100685No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT184600.41831750253120614No Hit
TCCCTGAGACCCTTTAACCTGCCGCCTTGGCCGTA175120.3968351085767325No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC174400.3952035343523421No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC165030.3739704086821503No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA158610.3594222051813359No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT157570.3570654868572164No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC145210.3290567960051811No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC141870.321488104464259No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGCG136960.31036167468404113No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA127550.28903790600138324No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC122410.2773902788994851No Hit
GCGCCGCTGGTGTAGTGGTATCATGCCGCCTTGGC121120.2744670417474523No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA121030.2742630949694035No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC120840.2738325406601893No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA118720.26902846099948424No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA111280.2521688606807834No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG110220.24976682085043087No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA110170.24965351708484818No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT108060.24487209817725966No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA105680.23947883893552474No Hit
CAACGGAATCCCAAAAGCAGCCCGCCTTGGCCGTA104590.2370088168458226No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG104320.23639697651167615No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA103550.23465209852170313No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC101470.22993866187346418No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG97330.22055711008321938No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG97100.22003591276153908No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA96030.21761121217807No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT95320.21600229870679613No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCCAGATTG91850.2081390173753591No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA90990.20619019260733717No Hit
ACTGGACTTGGAGTCAGAAGGCCGCCTTGGCCGTA83850.19001041488213236No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG83370.18892269873253875No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC82910.18788030408917822No Hit
TCCCTGGTGGTCTAGTGGTTAGGATCCGCCTTGGC81910.18561422877752487No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGCGCCTTGG75190.17038620268321447No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT75170.1703408811769814No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG75040.17004629138646646No Hit
GCGCCGCTGGTGTAGTGGTATCATGCTATTCCAAT73710.16703241122196752No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA72290.16381458427941978No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG70890.1606420788431051No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA67590.1531640303146491No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA66990.1518043851276571No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT66020.14960629207535336No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC65290.14795205709784642No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT64300.1457086425393096No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT59460.13474083803090747No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCCG58070.13159099334770932No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA58030.13150035033524318No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC56330.12764802230543248No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG55560.12590314431545946No Hit
TACCCTGTAGATCCGAATTTGCCGCCTTGGCCGTA54920.12445285611600129No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT54890.1243848738566517No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCCG54480.12345578297887384No Hit
TGACCTATGAATTGACAGCCAGCCGCCTTGGCCGT54240.12291192490407703No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC54220.12286660339784397No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC53620.12150695821085196No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC53610.12148429745773542No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA53010.12012465227074343No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG52370.11867436407128529No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC51820.11742802264987597No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT51690.11713343285936102No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA51340.11634030650028236No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT50680.11484469679459115No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC50060.11343973010136607No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA49490.11214806717372368No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT49170.11142292307399461No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCCG49090.11124163704906234No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA48690.11033520692440099No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47130.10680012943822181No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT45030.1020413712837498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)