FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266789
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1922648.481777527595202No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA859073.789810167598308No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT366031.6147510862281402No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA365481.6123247465908825No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT352221.5538279036999032No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA319231.4082916407305663No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA296511.3080617560787529No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA285251.2583879664141655No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN273701.2074348340317516No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250011.1029257685651377No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT209120.9225384453515524No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC192770.8504099852257974No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT186510.8227938286271903No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA177640.7836635875681416No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA174920.7716642351802484No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT153510.6772134503917215No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA111890.4936057127505031No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT98380.4340059882062247No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA98270.4335207202787732No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93350.41181600934184875No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA88500.39042010526784804No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT80710.356054312951051No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA78890.34802533451503426No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA71080.3135713116659733No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA70790.3122919689481465No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69430.3062922927541999No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT68130.30055730815704507No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA68030.30011615549572546No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC66110.2916460243983891No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA63830.28158774372030215No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT61300.27042658138891623No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA60670.2676473196226027No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA55930.24673668347605357No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC53430.23570786694306353No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA52120.22992876707977672No Hit
TCGATTCCCGGTACCCGCTCCACGCCTTGGCCGTA48920.21581188191754944No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA48740.21501780712717417No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT47240.20840051720738012No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT46260.20407722112644805No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA45740.2017832272875861No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA43060.18996033596422077No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTC40470.17853448203604305No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT40160.1771669087859523No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC39960.17628460346331307No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA38000.16763801130144887No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG37350.16477051900287149No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA36730.16203537250268993No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC36380.16049133818807132No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT36170.15956491759930017No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC36070.15912376493798056No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA33800.14910959952602557No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT31280.1379925524607716No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC31200.13763963033171592No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC31140.13737493873492415No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC30730.13556621282351378No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT29420.129787112960227No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG29360.12952242136343523No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA29100.12837542444400427No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG28760.12687550539551762No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC27940.1232580535726969No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA27170.11986117808053594No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA26880.11858183536270911No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA26600.11734660791101423No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC26490.11686133998356266No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA25930.1143908850801729No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA25830.11394973241885327No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT25800.11381738662045739No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA25740.11355269502366563No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA25210.11121458591867174No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC24990.11024405006376861No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC24920.1099352432008449No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA24630.10865590048301804No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC23120.10199449529709205No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA22730.10027399991794561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)