FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4393966
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3577438.141687942055082No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1807454.1134819887090615No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1502043.418415162975772No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA741191.6868359928137817No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA701711.5969855023912338No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN662341.5073853552804004No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN597301.3593641826086047No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA581461.3233147457217467No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT576041.310979647999097No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT531081.208657508956601No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA452521.0298668674268303No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT416390.947640468770127No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA415470.9455466883448803No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA402770.9166434150833211No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC361060.8217177829778383No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA343110.7808663062026424No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN331580.754625775438408No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA323540.7363279552003816No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN312140.7103832847136277No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA303640.691038574262978No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT290800.6618166822410552No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA279540.6361906305146648No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC248690.5659807108202476No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA210050.4780419329598818No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT166760.3795204605588664No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA158140.3599026483136192No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC152840.34784065238556694No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG150930.3434937821548915No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA137910.3138622374410726No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA135020.30728503588785167No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA130440.29686165072738385No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA120330.2738528245325521No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT115830.2636115072351493No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT110920.25243709213953863No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT108890.2478171201142658No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA107140.2438343856097202No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT105970.2411716431123955No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC101630.23129446154112254No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA101120.23013377891408351No Hit
GTCCAGTTTTCCCAGAGGCTTTTATTCCAATTACG99190.22574139171764188No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT93700.2132469846148104No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC93080.21183595867605715No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA92050.209491834939096No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA86060.1958595036921087No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTG85880.19544985100021253No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT85420.19440296078758917No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA85420.19440296078758917No Hit
ACTTGACCCCTTTGAAGGCTTTCTATTCCAATTAC83460.18994229814249813No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC82610.18800782709743316No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA80540.18329682114062784No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC78240.17806237007751083No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC78180.17792581918021214No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG77250.1758092802720822No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTC75580.1720086136306016No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC74290.16907276933867946No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC73940.16827622243777035No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC72590.16520382724854948No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA70090.15951420652777012No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC69840.1589452444556922No Hit
CCACTACGCCTTGGCCGTACAGCAGAAAGCTTGAC69350.15783007879441943No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA68880.1567604300989129No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT67790.1542797554646531No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT65420.14888599502135427No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC64450.1466784221816919No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT64310.14635980342132826No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA63660.1448805020339256No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA63520.14456188327356198No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC62330.141853623810471No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG62190.14153500505010735No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA61070.13898605496719818No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT60300.13723365178519817No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA58960.1341840150788604No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC58870.13397918873291237No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC58420.13295505700317206No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA57760.13145299713288633No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA57220.13022403905719798No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC56280.12808474166618494No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA55790.12696957600491218No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG53640.12207650218504194No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC52960.12052892534898997No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA52380.11920893334176913No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC51680.11761583953995092No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT51110.11631860601561324No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT50060.1139289653128859No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT49470.11258621482278196No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT48880.11124346433267804No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA47440.10796624279750912No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA47070.10712417893083377No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA46170.10507591547135323No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG46050.10480281367675581No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA45960.10459798733080773No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC45690.10398350829296359No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA45180.10282282566592459No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)