FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467502
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1117463.222665769190616No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN585311.6879874907065662No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC498831.4385860484002604No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA438601.2648875184498813No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN393241.13407288589884No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA329160.9492712621362583No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA324050.9345344285309714No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC323760.9336980915944678No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT322140.9290261404319304No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA316440.9125877937489294No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN303490.8752410236533389No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT282200.8138423568320942No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT262120.7559332337803987No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA261400.7538568110414933No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC235000.6777213106149614No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN227330.6556016406046774No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC211880.611045069332332No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA211290.6093435562546179No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA189240.5457531098756396No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC172620.497822351652573No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACCA163460.47140564014094294No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA158280.45646693210270683No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC155600.4487380252412255No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC145240.41886060916475315No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA130060.3750826964194974No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT124760.35979791792477694No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA124330.35855783212237513No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC104390.301052457936578No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA103830.29943746247298486No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA101200.2918527516350387No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA99370.2865751771736541No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA96730.2789616271310009No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA96570.27850019985568863No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC94840.27351101744137424No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACTG92330.2662723770599123No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA91710.2644843463680771No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC87890.2534677701699956No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC87510.2523718803911288No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA87270.25167973947816036No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA86930.25069920651812166No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA83370.2404324496424227No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT79800.23013685356201669No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA78890.22751248593367793No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT77470.2234173188652811No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA75870.21880304611215798No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA75390.21741876428622103No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA73180.2110453000459697No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA71530.20628683126931147No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC71040.2048737102386675No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC64580.1862435839979328No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA62410.17998547657650954No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA61030.17600566632694084No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA60950.17577495268928467No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT59900.1727468361950476No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA59390.17127603675498962No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC58000.1672673873007139No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA57220.16501792933356635No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG57110.16470069808178914No Hit
AACCGGGCGGAAACACCACGCCTTGGCCGTACAGC53440.15411670995431292No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG53290.15368412188370764No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG52310.15085787982241972No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC50420.145407270132793No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA48550.14001433885258033No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT48520.13992782123845926No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA45620.13156445187342358No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT45510.13124722062164637No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA44680.12885356663096373No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA43310.12490259558610205No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA42180.12164376545420882No Hit
GAAAATTACGAGAACAACATAAGGTTAATGCACTA41790.12051903647063505No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC41290.11907707623528406No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT41260.11899055862116302No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC40880.11789466884229627No Hit
ATCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGC40640.11720252792932778No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC40620.11714484951991376No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA37940.10941594265843249No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG37790.1089833545878272No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT37360.10774326878542535No Hit
ATCACATTGCCAGGGATTCGCCTTGGCCGTACAGC36920.1064743437783165No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA36140.10422488581116895No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC36130.10419604660646194No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG35990.10379229774056366No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC34820.10041811078984238No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA34760.10024507556160026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)