FastQCFastQC Report
Wed 8 May 2024
EGAF00002406331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364278
Total Bases117.7 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT57236317.012951961758212No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2970238.828729373731898No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC1005642.9891703361018322No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA462701.3753322406769No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN460181.3678417776414435No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC394811.1735355996145385No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA381801.1348645979910101No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG292250.8686856436953189No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN289980.861938282151475No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA279350.8303416067281003No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT247220.7348382030260282No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT223080.6630843229958999No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA200130.5948676060658483No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA182640.5428802257126194No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA182620.5428207775932905No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148590.4416698025549613No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA147440.4382515356935426No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA144810.4304341080017763No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCTAC143370.4261538434100868No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC141120.4194659299855719No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126750.37675245624767034No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC123260.36637875942475623No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA117480.34919825293866913No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA110800.32934258108277614No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG110190.32752941344324105No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT108750.3232491488515515No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG101080.30045079508887196No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA100740.29944017706027865No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA95950.2852023524809781No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC93400.27762271726652793No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA92640.2753636887320251No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG91750.27271824742188366No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA90840.2700133579924132No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT88330.2625526190166211No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA86170.2561322221290868No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA85380.25378402141559053No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA79840.2373168923614517No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT76520.22744850455283422No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA76140.22631899028558283No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA70410.2092871040978183No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC69890.20774145299526378No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC64560.19189852919407968No Hit
GTCCAGTTTTCCCAAAGGCTTTTATTCCAATTACG63020.18732102400574507No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC60190.1789091151206886No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA59590.17712567154081796No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC59200.17596643321390207No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC53860.16009378535305346No Hit
GTCCAGTTTTCCCAGGAATCGGAGCGGAACCGGCA52990.15750779216224106No Hit
GTCCAGTTTTCCCAGGAATAAAATAAGGTTAATGC51130.1519791170646421No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC48090.14294300292663092No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA43910.13051834598686554No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG43690.1298644166742463No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC41510.12338457166738302No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC41430.12314677919006693No Hit
TGGAATGTAAAGAAGTATGTATCGCCTTGGCCGTA41420.12311705513040243No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA41220.12252257393711222No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA40700.12097692283455767No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA39530.11749920785380993No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT38540.1145565259470234No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA38180.11348645979910102No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT37550.11161384404023687No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT37200.11057350195197899No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT36580.10873061025277934No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT36490.10846309371579875No Hit
TCCCTGAGACCCTTTAACCTGCGCCTTGGCCGTAC36020.10706606291156676No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC35630.10590682458465085No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA35570.10572848022666378No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC35290.10489620655605751No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC35120.10439089754176081No Hit
TGAGATGAAGCACTGTAGCTCGCCTTGGCCGTACA33910.10079428632235506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)