FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764235
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2157967.806716867415398No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA792942.868569423366682No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA438321.5856828381089163No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT346561.253728427575803No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN306781.1098188106293423No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN291361.0540348414660836No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA281931.0199205205056734No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA266070.9625447908734243No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC265570.9607359721586624No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT213780.7733785296836195No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC210820.7626703228922288No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA172320.6233912818555586No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT170880.6181818839570441No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA169080.6116701365839011No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139060.5030686609495937No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT134410.4862466469023075No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT128730.4656984663026118No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA122390.4427626449994302No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC120360.4354188410174967No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC119020.4305712068619347No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGTGGT118370.42821974253274414No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA106650.385821031858724No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC99690.36064227534923765No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT94390.341468796972761No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA91990.33278646714190363No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87860.31784562455796994No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA86600.3132874013967698No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA85350.30876535460986493No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA83290.30131302150504574No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC79410.28727658827849295No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA69790.25247491620647305No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA67010.2424178841523966No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC66930.24212847315803468No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA66550.24075377093481562No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC62260.22523410636215807No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA60390.2184691243689484No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT58370.2111614967613101No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGTTTG58260.21076355664406246No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT58030.20993150003527197No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA55690.20146622845018605No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA54880.19853594213227166No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT54420.19687182891469068No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA52950.19155390189329055No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA52620.19036008154154765No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA49780.18008599124169977No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA49490.17903687638713786No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA48620.17588953182345207No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGACCA48140.1741530658572806No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT45220.1635895645630708No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG45150.16333632994300412No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC44660.16156368760253742No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC44210.15993575075925165No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA43200.15628193695543252No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT43050.15573929134100392No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA42950.15537752759805154No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG41530.1502404824481276No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA41190.14901048572208947No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC41030.14843166373336564No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT40340.14593549390699415No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC40240.14557373016404176No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA39040.1412325652486131No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC38280.13848316080217493No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG37530.13576993273003202No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC36970.1337440557694986No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC36520.13211611892621286No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT34750.12571290067595553No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA33980.12292731985522215No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT33500.12119085388905067No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC33090.11970762254294588No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT32910.11905644780563157No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA32690.11826056757113632No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT32660.1181520384482506No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC32620.11800733295106965No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT32310.11688586534791723No Hit
ATCCCGGACGAGCCCCCACGCCTTGGCCGTACAGC32020.1158367504933553No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA31870.11529410487892672No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA30840.1115679383265171No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA29800.10780559539981226No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT29650.10726294978538366No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC29240.10577971843927887No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC29160.10549030744491696No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA29030.10502001457907884No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA28480.1030303139928407No Hit
TGACCTATGAATTGACAGCCGCCTTGGCCGTACAG28400.10274090299847878No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG28340.10252384475270734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)