FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4869988
Sequences flagged as poor quality0
Sequence length35
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT106954621.96198430057733No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA59212112.158572053976313No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC1667673.424382154535083No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN845311.7357537636643046No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG644561.3235350887928266No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT632681.2991407781702953No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA480880.9874356979935063No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA403290.8281129234815363No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN395800.8127330087877014No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT371460.7627534195156127No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA370090.7599402709000516No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA363180.7457513242332425No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG271680.5578658510041503No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA236410.48544267460207297No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA205270.4215000119096803No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN201410.4135739143505076No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG201000.4127320231589893No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA170050.34917950516510515No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA153050.3142718216143448No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT150670.3093847459172384No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142820.2932656096893873No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG141320.29018551996432024No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC138730.28486723170570444No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC129940.26681790591681126No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC128470.26379941798624557No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG119190.24474392955383054No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC115390.23694103558366056No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG110670.2272490199154495No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA109480.22480548206689627No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA107190.22010321175329386No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC104900.21540094143969146No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA101610.20864527797604426No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT100450.20626334192199244No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA98960.2032037861284258No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT98540.202341361005407No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT97940.20110932511538016No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC97670.2005549089648681No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA94870.19480540814474287No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT81170.16667392198913014No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT79080.1623823303055367No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA78590.16137616766201476No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG77000.15811127255344365No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC70570.1449079545986561No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT69590.14289562931161226No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC68400.14045209146305904No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC56530.11607831477202818No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC55810.11459987170399598No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT54930.11279288573195663No Hit
TCCCTGAGACCCTTTAACCTGTCGCCAACCGGCAT52900.10862449763736584No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA52140.10706391884333186No Hit
ATCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT51820.10640683303531755No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA50770.10425077022777059No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC49030.10067786614669277No Hit
CACGCTCATGCACACACCCACCGCCTTGGCCGTAC48950.10051359469468919No Hit
TCCCTGAGACCCTTTAACCTGTGACGTTCCAATTA48810.10022611965368293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)