FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680975
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3219166.877114276406091No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1391722.9731412793274905No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT901541.9259662783928562No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA681641.456192353088833No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN658611.4069932012027409No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT613941.311564364261719No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA590781.2620874924561658No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA508461.0862266942250278No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT493971.0552716047404653No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN476241.0173948803401No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA423710.9051746698070381No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT421650.9007738772371141No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT409650.8751381923637703No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA383810.8199360176031703No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC279510.597119189912358No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT240500.5137818510032632No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240410.5135895833667131No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA223950.4784259689487767No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT214160.45751152270627377No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA201790.43108540421600205No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA174470.37272149498768953No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN158430.33845512954032014No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT153310.3275172373276935No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT151540.3237359738088753No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA150250.32098013768499084No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA139330.29765166445024804No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC129600.2768653966321119No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT121620.25981766619133834No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA116270.24838842335197261No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT113040.2414881515068976No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG109660.23426743360090582No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA99250.21202847697328014No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC99040.21157985248799663No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA97710.20873856408120103No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC92310.19720250588819635No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG89660.19154129214533297No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA88900.1899176987700212No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA86220.18419239581497446No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT85490.18263289165184604No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG85400.18244062401529595No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC84950.18147928583254555No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA77500.1655637981403447No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG72800.15552315489828508No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC72570.15503180427154598No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC71700.15317321711822857No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT68580.14650793905115922No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA65650.1402485593279178No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA63480.13561277297998814No Hit
TGTGCAAATCCATGCAAACAGTCATAAGGTTAATG62360.13322010905847606No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA62180.13283557378537592No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC62010.13247240158300355No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAC61160.1306565405711417No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT59130.12631983721340106No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC54910.11730462136627519No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC54680.1168132707395361No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA54340.11608692633479137No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC54320.11604420019333578No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC53350.11397198233274052No Hit
TGTGCAAATCCATGCAAACAGTATAAGGTTAATGC52760.1127115611598011No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC52690.1125620196647066No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA52270.11166477069413958No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA51750.11055389101629469No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG51600.11023344495537789No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC51470.10995572503591666No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT51350.10969936818718323No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA51280.10954982669208872No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA47800.10211547807881904No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA47700.10190184737154119No Hit
TAGCACCATCTGATTAGCCAATGCGGAACCGGCAT47600.10168821666426332No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA47540.1015600382398966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)