FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707747
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2032387.505797255061126No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1129074.169776570706199No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT461951.7060308810239657No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA318031.1745188896894725No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA314531.1615930144138282No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN312861.155425525353735No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC312341.1535051095984965No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN296671.0956341194358261No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT261620.9661907113183026No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA248980.9195098360371187No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA224810.8302474344907409No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT213140.7871488732145212No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA211840.7823478338264247No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC197440.7291670898352025No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT180950.6682677517508098No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT154220.5695509957171035No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN152690.5639005416680362No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC150140.554483118252924No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA123510.45613567294137897No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122250.45148235784214696No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA111020.41000876374343687No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA107790.39808002741762805No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA105790.39069381297440264No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACCTA100240.3701970678944525No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC99600.3678334792726204No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC97460.3599302298183693No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA94160.3477429759870475No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA92000.3397658643883642No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC86180.31827198035857857No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC80750.2982184081452218No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA75000.2769830416209491No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG73450.27125872542744944No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA72700.26848889501123996No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA70320.25969929982380185No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT66390.24518538844286414No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT63220.23347823855035202No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT62690.2315208917228973No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA61800.22823402629566203No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT54280.2004618599891349No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA52090.19237395517380315No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC51890.19163533372948063No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA50420.18620646611371003No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA50380.18605874182484552No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACCTA49090.1812946335089652No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA48650.17966966633145562No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT47750.17634586983200426No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACTCG46170.17051076042185626No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG44640.1648603063727889No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA44170.16312454597863094No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT44100.16286602847311807No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG43770.16164730308998587No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT43340.16005926698469244No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACCTCACC42770.15795419586837323No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC42490.15692012584632167No Hit
CGTACCGTGAGTAATAATGCGACGCCTTGGCCGTA41770.15426108864676058No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC41700.15400257114124768No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACCTA40920.1511219475083898No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAA38240.1412244201544679No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC37730.13934093547144544No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA37080.13694041577739724No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC36860.13612793218864244No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG36410.13446603393891676No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC36090.1332842396280007No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA35870.1324717560392459No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC35600.1314746170894105No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA35040.12940647704530742No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT34810.1285570623843365No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG34740.1282985448788236No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA34270.12656278448466568No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT33570.12397760942953681No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA33520.12379295406845618No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC32710.12080153721894993No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG32680.12069074400230155No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32180.11884419039149521No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACC31910.11784705144165979No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC31690.11703456785290502No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA30640.11315680527021173No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC29840.11020231949292161No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACTCG29460.10879893874870879No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT29170.10772793765444114No Hit
TCAATCCCCGGCACCTCCACCACGCCTTGGCCGTA29070.10735862693227988No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC28760.10621376369357993No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA28410.10492117616601551No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC28190.10410869257726073No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACC28010.10344393327737045No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC27950.10322234684407368No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)