FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505139
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC612022.4430580498726817No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA561072.2396761217641017No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN350331.398445355726768No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT318731.2723046505603082No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA249790.9971103399851265No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA228210.9109674153809428No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA209330.8356023358384504No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT199890.7979197960672042No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198050.7905748942473851No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC156340.6240771470165927No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA134460.5367366840722212No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133610.533343658775022No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129710.5177756603525793No Hit
CTGACCTATGAATTGACAGCCCGCCTTCGCCGTAC123910.49462325244227967No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA122090.48735818651180635No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT101030.4032909950306151No Hit
CGCCTTGGCCGTACAGCAGCCTTTAACGAAGAGAA95680.3819348946305973No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA91830.3665664859315192No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA88520.3533536462447792No Hit
TAGCACCATCTGAAATCGGTTACGCCTTCGCCGTA85470.3411786731195355No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA68970.2753140644092004No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC67390.2690070291508775No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTCGCCGT66300.26465597318152806No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC64910.2591073788719907No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT64620.2579497584764757No Hit
TAGCACCATCTGAAATCGGTTCGCCTTCGCCGTAC62170.24816986203160782No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC58760.2345578428981386No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA58120.23200309443907105No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC55030.2196684495351356No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT54830.21887009064167698No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA54050.21575649095718843No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA53920.2152375576764403No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGCCGTA51790.20673503546110614No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTCGCCGTA49780.19871152858184715No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA48150.19220490360015952No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA47950.1914065447067009No Hit
ATCACATTGCCAGGGATTTCCACGCCTTCGCCGTA47060.18785384763081012No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTCGCCGT46170.1843011505549193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG45440.1813871405937954No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA45110.1800698484195887No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT45030.17975050486220526No Hit
TAGCACCATCTGAAATCGGTTACGCCTTATAATGC42050.167854957349672No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA40800.16286521426555572No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG39360.15711703023265375No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA39090.15603924572648462No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC36870.1471774620090941No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA35910.1433453393204928No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35600.14210788303563196No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC33540.1338847864330083No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA33110.1321683148120723No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT32450.1295337304636589No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC32170.12841602801281687No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC31590.1261007872217869No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG30440.1215102235843999No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA30270.12083161852496009No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA29800.11895547512533236No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT28860.11520318832607691No Hit
GCTCCCCGTCCTCCCCCCTCCCGCCTTGGCCGTAC28830.11508343449205813No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT28650.11436491148794539No Hit
CTGACCTATGAATTGACAGCCGCCTTCGCCGTACA28060.11200975275224248No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC27850.11117147591411096No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA27680.11049287085467113No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG27440.10953484018252083No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA27180.10849697362102463No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC26900.10737927117018257No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCA26320.1050640303791526No Hit
CGCCTTCGCCGTACAGCAGCCTTTAACGAAGAGAA26300.10498419448980675No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG26180.10450517915373159No Hit
TGACCTATGAATTGACAGCCAGCGCCTTCGCCGTA26030.10390640998363762No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA25940.10354714848158125No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC25430.10151133330326181No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC25100.10019404112905511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)