FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196921
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1281554.008700871870152No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT819492.5633726951651292No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA758992.3741281063873645No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN626311.9591037751636655No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT413741.2941827464613607No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA399371.2492332466144769No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN375891.1757875781103129No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT340101.063836109806905No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA330841.0348707396898453No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA274830.8596709146081495No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT242060.7571660356949702No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA241030.7539441856711505No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA238740.7467810433851821No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA222480.6959196051450756No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA219460.6864730157548465No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN209670.6558498004798993No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200590.6274474721145753No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA199470.6239441012148876No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA185910.5815282892508135No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC184070.575772751344184No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC164280.5138694387505979No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT162070.5069565372431787No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA158900.4970407463931702No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA156350.48906432157691726No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA141730.44333281929706736No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA139480.4362947974003737No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA132950.4158688938513026No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA132090.41317880548189967No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT125600.39287802232210306No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA107240.3354477636450822No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGCGGCATGTC104590.327158537855643No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC103750.32453100968087734No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA96940.30322926340688433No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA94890.29681684345656334No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC84300.2636912203961249No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC81370.25452615188176375No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA80590.2520863042909099No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA78050.24414115957197566No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA77560.24260843480336233No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTG77560.24260843480336233No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC77050.2410131498401118No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT75250.2353827323227568No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA72190.22581102254325336No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT70360.22008676473394242No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT69740.21814739870018685No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA66810.20898233018582568No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC63520.19869117816799353No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC62590.1957821291173601No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTC61840.1934361218184622No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT61080.19105883442224564No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC60540.18936970916703916No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT59310.1855222571968466No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG58900.18423977320678242No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA57820.1808615226963694No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA55920.17491830420582805No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA54460.17035140999730677No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT53500.16734852065471748No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT52890.16544043471828052No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG52770.16506507355045683No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC51840.16215602449982344No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA48120.1505198282972898No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC47000.14701645739760225No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC46830.1464846957431854No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC46340.1449519709745721No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA45790.14323156562204697No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT44920.1405101971553254No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA43940.13744474761809877No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA43260.13531770100043133No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA43020.13456697866478404No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA41800.1307508067919101No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA41030.1283422392983749No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC39190.12258670139174536No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA37700.11792596689126819No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA37510.11733164504221405No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC36810.11514203822990934No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA36590.11445387608889929No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC36160.11310883190419783No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC35580.11129458625971676No Hit
TAGCACCATCTGAAGCTAACCTATTCCAATTACGT35360.11060642411870672No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA35100.1097931415884221No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC34910.10919881973936796No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA34570.10813529643053424No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG34120.1067276920511955No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT34070.10657129156460231No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTT33570.10500728669867038No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG32530.10175415657753195No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)