FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911172
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1163273.995882070863556No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA748952.5726751974806024No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN579151.9894049544307242No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT534251.835171539160173No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA366741.2597675437933589No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN345121.185501921562862No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT277330.9526403798882375No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT277090.951815969650711No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA265870.9132747910463552No Hit
CTGACCTATGAATTGACAGCCCGCCTTCGCCGTAC231130.7939414091644189No Hit
CCACTACGCCTTGGCCGTACAGCAGCCTTTAACGA224910.7725754438418616No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA221790.7618581107540193No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA214530.7369197010688479No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA203240.6981380694785467No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT199360.6848101039718711No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN192690.6618983694539519No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC183020.6286815069669536No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN171540.5892472172719441No Hit
TAGCACCATCTGAAATCGGTTACGCCTTCGCCGTA163480.5615607734616848No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA158300.5437672525017416No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA127170.43683437460926394No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTCGCCGT126430.43429244304355774No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT116510.4002168198924694No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT105910.36380536773505656No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA100490.3451874365375869No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTCGCCGT99630.34223329985311757No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA98890.3396913682874114No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA95810.3291114369058235No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGCCGTA86990.29881436067673084No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC81900.28132999355585997No Hit
CGCCTTGGCCGTACAGCAGCCTTTAACGAAGAGAA80970.27813540388544544No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA78280.2688951391398378No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT77750.26707456653196715No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCGCCGT70930.243647575615594No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC69210.23773930224665532No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTCGCCGT68950.23684619115600178No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA68470.2351973706809491No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA68170.2341668578840412No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA66110.22709067001194022No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC64590.22186940517427345No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG61570.21149557635206714No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT59940.2058964568222008No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA59040.2028049184314771No Hit
CCACTACGCCTTCGCCGTACAGCAGCCTTTAACGA57950.19906072193604502No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG57390.19713709804848356No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA56430.19383945709837827No Hit
CTGACCTATGAATTGACAGCCGCCTTCGCCGTACA55180.18954565377792862No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG54700.18789683330287596No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTCGCCGTA53080.1823320641995732No Hit
TAGCACCATCTGAAATCGGTTCGCCTTCGCCGTAC52660.18088934628390216No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG52330.17975578220730343No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA51120.1755993805931082No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTCGCCGTA50870.1747406199290183No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA49790.1710307738601498No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG49190.16896974826633396No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC49120.16872929528038877No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTCGCCGT49020.16838579101475282No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA48280.16584385944904664No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT47750.164023286841176No Hit
TGACCTATGAATTGACAGCCAGCGCCTTCGCCGTA47520.16323322703021326No Hit
GAGCCCAGCGCCGAATCCCCCGCCTTGGCCGTACA47220.16220271423330535No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG47210.16216836380674177No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG46170.15859591944412765No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA46050.1581837143253645No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTATTAC46020.1580806630456737No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA45750.15715320152845658No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTCGCCCA44840.15402731271116923No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTTAATG43430.14918390256570205No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA43370.1489778000063205No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTCGCCGT42720.14674502227968667No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA40580.1393940309950769No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG38590.1325582961089211No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA38140.13101252691355922No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA38040.13066902264792324No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA37400.12847059534785302No Hit
ATCACATTGCCAGGGATTTCCACGCCTTCGCCGTA37220.12785228766970827No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT37170.1276805355368903No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC37040.12723397999156355No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA35760.12283712539142311No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC35690.12259667240547792No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT35620.12235621941953274No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG35600.12228751856640556No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC35570.12218446728671475No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG35390.12156615960857002No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC34500.11850897164440988No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC34030.11689450159592081No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA33930.11655099733028484No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTCGCCGTA33580.11534873240055894No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC33500.11507392898805018No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG33110.11373426235206988No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA32680.11225719400983522No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA32470.11153583505199968No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT32080.11019616841601938No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA30600.10511230528460704No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA30080.10332608310329998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)