FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812889
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1729376.148020771527067No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA842702.99585230700536No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT734402.610838891971919No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA384461.3667798480494608No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA373911.3292739244243197No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT358461.2743481879306293No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN354021.2585637044334135No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA312071.1094287758955295No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA309581.1005766669072259No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA306941.091191298341314No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN304621.0829435502076334No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT294111.0455798291365213No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA213370.7585439738290419No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC202630.720362588072263No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC146430.520567999661558No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142080.5051034719109073No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA140380.499059863364676No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA126080.4482224502993186No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT123590.4393703413110151No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA123550.43922813875698613No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119530.4249367820770745No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGTGGT119050.4232303514287269No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA114420.40677040579987334No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT113460.40335754450317807No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA104970.3731750524105288No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA81560.2899510076650732No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC77780.27651286630933536No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT76500.2719623845804083No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT75490.26837177009117674No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT67010.2382248286370347No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA65810.23395875201616556No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC63270.22492888983532588No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGTTTG62330.22158712981564505No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT61780.2196318446977467No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC61520.21870752809655838No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA61090.21717885064074693No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT60240.21415704636763128No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA59030.2098554191082549No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT58320.20733132377424063No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC55180.19616842328296635No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA54970.1954218598743143No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA53600.190551422398822No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA50170.1783575533908377No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG49990.1777176418977073No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA49740.17682887593502625No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA47630.169327691209998No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC47000.16708800098404167No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT46170.16413729798794052No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC45010.16001342392110035No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT44980.1599067720055786No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC43990.15638725879336157No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43950.15624505623933257No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA43080.15315215068920246No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG42010.14934823236892747No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC41880.14888607406833332No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT39320.13978511061047913No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT39170.13925185103287047No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT38850.1381142306006387No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC38080.135376831435581No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC36720.13054194459859597No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA36610.1301508875750163No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35310.1255293045690747No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC35250.12531600073803126No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT34330.12204534199536492No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG33760.12001895560045206No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA33660.11966344921537962No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG32800.11660609430375674No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT31390.1115934542742355No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC30560.10864275127813433No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA29460.10473218104233761No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC29260.10402116827219275No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT29200.1038078644411493No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA29160.10366566188712031No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT28850.1025635920933958No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC28660.10188812996175817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)