FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541801
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT489941.9275309121367095No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC483201.9010142808189938No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA332121.3066325805993466No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA271841.06947790169254No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT259541.021087016646858No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA235360.9259576182399802No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC221660.8720588275793424No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT211900.8336608570065084No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC211130.8306315089182827No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA200890.7903451135631783No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA175700.6912421546769397No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT162720.6401760011897076No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT161070.6336845410006526No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA124270.48890530769324586No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA118880.46769987107566646No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT99510.391494062674458No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT94200.3706033635205903No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC93040.3660396702967699No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA84670.3331102631559276No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC83720.32937275577435055No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA81590.32099287080302513No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA80260.3157603604688172No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA79390.3123375905509519No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG68280.26862842527798203No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA66670.262294333820783No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT65630.2582027467925302No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA59240.23306309187855384No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC59070.23239427476816635No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56250.22129977917232702No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC55630.21886056382856095No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG55070.21665740158257865No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA53230.20941843991720832No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT53170.20918238681942447No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA49850.19612078207538672No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC49460.1945864369397919No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC49080.19309143398716108No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT48940.19254064342566551No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA47320.1861672097855025No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46980.1848295755647275No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT46380.18246904458688937No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT45430.1787315372053123No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC44300.17428587053038377No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA43270.17023362568509495No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC43140.16972217730656336No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG42160.16586664337609436No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA41740.16421427169160766No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC40720.16020136902928278No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA40250.1583522864299762No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC38800.15264766990020068No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA38510.15150674659424557No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA38440.1512313513134978No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC37420.14721844865117292No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG37240.14651028935782148No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC36480.14352028345255982No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC35670.1403335666324783No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA35630.1401761979006224No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC33970.13364539552860355No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA32310.12711459315658463No Hit
CTCCTGACTCCAGGTCCTGTGTCGCCTTGGCCGTA31600.12432129816614282No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG31500.12392787633650312No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31260.12298366394536787No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG30820.12125260789495322No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG30580.12030839550381796No Hit
TAGCACCATCTGAAGCTAACCTATTCCAATTACGT30540.12015102677196209No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA30470.1198756314912143No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA29880.11755444269634012No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC29610.11649220375631293No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA29270.115154569535538No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC28940.11385627749772702No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA28680.1128333807406638No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA28220.11102364032432122No Hit
CTGACCTATGAATTGACAGCTGTATTCCATAATGC27550.10838771406573527No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG27500.10819100315091544No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA27300.10740415949163605No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC26800.10543705034343757No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT26110.1027224397189237No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)