FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986560
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN468351.5681921675774135No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAC362081.2123647273116898No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT346031.1586239687131683No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN327421.0963114754098362No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN268570.8992620272152576No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230680.7723936569163184No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAATTACG225690.7556854709096753No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTATAATGC186870.6257031501125041No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAATG174030.582710543233687No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCCGGCA172670.5781568091717562No Hit
CTGACCTATGAATTGACAGCCCGCCTTATAATGCA153020.5123620486445944No Hit
CTGACCTATGAATTGACAGCCCGCCTTAATTACGT151090.5058997642772957No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTATAATG129540.4337431693989071No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC123990.41515991642558664No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT118520.3968445301617915No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGCGGCA114220.38244669452480445No Hit
CTGACCTATGAATTGACAGCCCGCCTTAGCCGTAC113860.38124129433194043No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA112430.37645317689917496No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTAATTACG94710.31712070073931214No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTATAATGCA85470.28618209578913534No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG81250.2720521268616736No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT80950.2710476267009536No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA77370.2590605914496946No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTCAATTAC74910.2508236901317904No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTAATTACG74000.24777670631093968No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTTTAATG68720.23009750348226723No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA67330.22544331940426446No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCGCCGT65290.21861271831136825No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGCGGCAT61290.20521938283510127No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTAATTACGT59130.19798698167791706No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACGT58600.19621236472731168No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTAATTAC58330.19530831458266365No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGCGGCAT53430.17890147862423658No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTATAATGC52520.17585449480338583No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTTATTAC49080.16433622629379618No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAGCCGTA45860.15355459123540124No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA45800.15335369120325726No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGCGGCAT44700.14967052394728383No Hit
CTGACCTATGAATTGACAGCCGCCTTATAATGCAC42080.14089788921032895No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC41550.13912327225972357No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGCCGTA41180.13788438872816888No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTCGGCCGT39790.13323020465016608No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGCCGT38570.12914523732990463No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTTTAATGC36410.12191283617272045No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG36000.1205400192864031No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTATAATGC35330.11829663559412835No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA34930.11695730204650165No Hit
ATCACATTGCCAGGGATTACCACGCCTTAATTACG34220.11457998499946427No Hit
TAGCACCATCTGAAATCGGTTCGCCTTATAATGCA33860.11337458480660025No Hit
CTGACCTATGAATTGACAGCCACGCCTGATAATCG33330.11159996785599485No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTAATTAC32740.10962445087324547No Hit
CTGACCTATGAATTGACAGCCACGCCTTATAATGC32510.10885433408336011No Hit
CTGACCTATGAATTGACAGCCACGCCTGATAATGC31980.10707971713275474No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA31160.10433408336012No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)