FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271028
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN964282.257723433327995No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT795481.8625024233041787No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT723411.693760846334887No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA640941.500669159743275No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA589561.3803702527822344No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC479121.1217908194467467No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC478441.1201986968945181No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA464361.0872323946366074No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA450161.0539851295753622No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT449721.0529549326298024No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT434161.0165234224641No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG396200.9276455223426304No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA369960.8662083226801605No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT362140.8478989133295309No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN351060.821956681154982No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN331460.7760660899436856No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC312950.7327275775293443No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA276860.6482280144265034No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA245900.5757396111662111No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA243280.5696052566267419No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA221790.5192895012629278No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN213270.4993411422261807No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGACAC212420.4973509890358949No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA184730.43251882216646675No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC166820.39058512376879756No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA163390.3825542703068207No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA160670.37618578009790615No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA153590.35960897470117265No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA135750.3178391712721153No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT134150.31409300056098904No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA133410.3123603966070932No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT129840.3040017532078928No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA125240.29323151241340495No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA125090.29288030890923683No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA120290.2816417967758582No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA117940.2761396085438915No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA116830.2735407026130477No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA115230.2697945319019215No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG113550.26586105265523896No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA102570.24015295615013527No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC101250.23706236531345617No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA99460.23287133683038372No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG95490.22357615075340176No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC90270.21135426880835245No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA88010.2060628026788867No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCCA87830.20564135847388498No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC87070.2038619273861No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC80270.18794070186381356No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC78170.18302385280546044No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGGT76680.17953523133072413No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA74250.17384573456320118No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG73660.1724643341134734No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAACA73370.17178534067208176No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT73100.17115317436457922No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC67980.1591654280889753No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA67430.15787768190702567No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA66250.15511488100757007No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA66030.1545997825347902No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA64500.15101750679227577No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGAGTG64180.15026827265005052No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC63920.1496595199094925No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT63050.14762253958531762No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT61630.1442978130791931No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT60190.14092625943917952No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA58850.1377888414686113No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG55760.13055404928274877No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT55490.12992188297524626No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC53490.12523916958633846No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC50230.11760634676241878No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC48010.11240853490073117No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA45850.10735120444071075No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA45200.10582932258931574No Hit
AACCCGTAGATCCGATGAACACGCGGAACCGGCAT45110.10561860048681489No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG44520.10423720003708709No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA44490.10416695933625347No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT44320.10376892869819632No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT44120.10330065735930553No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA43940.10287921315430383No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC43920.10283238602041475No Hit
ATCACATTGCCAGGGATTTCCATCGCCTTGGCCGT43790.10252800965013574No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG42970.10060809716068357No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC42850.10032713435734911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)