FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305041
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC803772.4319516762424427No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA703702.1291717712427776No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN582261.7617330617078577No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN474521.4357461828764No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT451081.3648242185195283No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT364651.1033146033589296No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGATGT348161.0534211224611132No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT288600.8732115577386181No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC234620.7098852934048322No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA224850.6803243893192248No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA224410.6789930896469968No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN221890.6713683733424184No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGATGG221040.6687965444301599No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT216000.6535471118210031No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT214290.6483731971857535No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA213590.6462552204344818No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA212560.643138768929039No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA208550.631005787825325No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC197290.5969366189405819No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA190730.5770881511000923No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA161260.48792132987155074No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA159240.48180945410359505No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN158410.4792981388128014No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC156110.47233907234433703No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA153530.4645328151753639No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA151700.4589958188113249No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA138320.41851220605130157No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA138080.41778604259372276No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT134060.40562280467927625No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC128920.39007080396279503No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT125300.37911783847764674No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA111210.33648599215561925No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC106940.3235663339728615No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA101190.3061686678017005No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA100100.302870675431863No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT99640.30147886213817016No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC97670.29551827042387674No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA94850.2869858497973248No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA94830.28692533617585986No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA94610.28625968633974586No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA89530.2708892264876593No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC89510.2708287128661944No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA88110.26659275936365084No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA87610.2650799188270282No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA85810.2596336928951865No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAGCATG72250.21860545754197905No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA69550.2104361186442165No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGCGGCAT67240.2034467953650197No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA67090.20299294320403286No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA66610.2015406162888751No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC63300.19152561193643286No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT62730.189800973724683No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA60880.18420346373917904No Hit
TCTCCCAACCCTTGTACCAGTGCGGGAACCGGCAT58790.17787979029609619No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC57120.1728269029037764No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC56870.17207048263546504No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA55260.16719913610753998No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG54970.1663216885962988No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA54860.1659888636782418No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT54140.16381037330550513No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA53880.16302369622646135No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT53300.16126880120397902No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA51100.15461230284283917No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC50760.15358357127793573No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT50510.1528271510096244No Hit
TCTCCCAACCCTTGTACCAGTGCGAAGGTTAATGC49770.1505881470154228No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC49290.14913582010026502No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGTACA48940.14807683172462915No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT48290.14611013902701964No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGAGTG48140.14565628686603282No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT47930.1450208938406513No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC47100.14250957854985763No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCTACG46110.13951415428734468No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA45210.13679104132142383No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC44890.13582282337798532No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA43630.13201046522569615No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40270.12184417681959164No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC40010.12105749974054783No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT39320.11896977980000853No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC39250.11875798212488135No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA38030.11506665121552198No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGCGTG37250.1127066199783906No Hit
CGCCTTGGCCGTACAGCAGAGCCAAGTACAGAGTT37130.11234353824960114No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA35620.1077747598290006No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT35550.10756296215387344No Hit
TCTCCCAACCCTTGTACCAGTGCGGGAACGCCGTA35380.10704859637142171No Hit
TTCAAGTAATCCAGGATAGGCTCGGGAACCGGCAT34180.10341777908352726No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG34030.10296392692254044No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC33500.10136031595372039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)