FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458013
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT2130116.159924789178063No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC947772.740793629173748No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN530111.5329901882959953No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA433231.2528292982125864No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN409491.1841771560719985No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT391411.1318927950820312No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC304960.8818937349281221No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN281250.8133283478113009No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278440.8052022939185017No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC234630.6785110408780997No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA189520.5480604034744808No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT175760.5082687659068951No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT164560.4758802237007206No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA164460.47559104028816546No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA153130.44282655964566936No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT137450.39748260055702506No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA137110.3964993769543377No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC137050.39632586690680455No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC136930.3959788468117384No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA128160.37061746153065356No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG126910.36700266887371447No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA124920.36124791896386743No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA108390.31344590086850455No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA98490.28481674302554677No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA92600.2677838400260496No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC87180.25211009906556164No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGAAAC86900.2513003855104073No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA79970.23125997502033685No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA77100.22296041108000464No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA76570.22142773899346244No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC75490.21830455813786703No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG72310.20910852561861396No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA72130.2085879954760147No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT65860.1904561955088081No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG65570.18961756361239823No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT63400.1833422835599519No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA61260.17715375853127216No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA61100.17669106507118396No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA60280.17431976108823188No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT59410.17180386539900225No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA58810.17006876492367148No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG54990.15902195856406554No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT53890.15584094102595913No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC53550.15485771742327167No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGAAAA50380.1456906032452741No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA49340.14268309575470073No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC49130.142075810588335No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA47060.13608971394844382No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA46930.13571377551212213No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC45990.1329954514341039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)