FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201523
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1783874.2457699267622715No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1303503.102446422404447No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA961492.288432075702073No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA624831.4871512068361876No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA571121.3593166097150962No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN540041.2853434337976966No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT501341.193233977298232No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT497661.1844752486181795No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA416860.9921640319474628No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA378330.9004591906315875No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA377980.8996261593712566No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA328320.7814309239768532No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN303380.722071496454976No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA279620.6655205743250722No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC278520.6629024760783173No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA275830.6565000358203442No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT263120.62624910062375No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA252920.6019721896083873No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA241670.5751961848120313No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN220960.5259045350935839No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT205770.48975097839521525No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196650.4680445638403027No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT195770.4659500852428988No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA193430.46038067624525675No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC151850.36141656251792503No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT127070.30243794928648493No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA117880.2805649284795061No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA114730.2730676471365265No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC111680.2658083747250699No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT103060.24529200482777316No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA100810.239936803868502No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC97630.23236811984606534No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA96880.23058305285964162No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA92830.22094369113295342No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT91650.21813518574098015No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC91490.21775437145054305No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC89480.21297039192692746No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA88590.21085211243637128No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAT86850.20671075702786823No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT84500.20111754713707386No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG84210.20042732123565668No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA82770.19699999262172313No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA82580.1965477756518291No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT82580.1965477756518291No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA80500.1915971898761473No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC80480.19154958808984265No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA79750.18981212288972357No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA79560.18935990591982954No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG78460.18674180767307474No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC77210.1837666960290352No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC76620.18236244333304852No Hit
TAGCTTATCAGACTGATGTTCTGGCGAGGTTAATG74930.17834009239030704No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT74000.1761266093271416No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT73100.17398452894343314No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT69820.16617783598947333No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA69740.1659874288442548No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG67720.1611796484274869No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA66850.15910897072323535No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC63760.1517544947391696No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT63620.15142128223503715No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT63560.15127847687612325No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA63260.15056445008155378No Hit
TGACCTATGAATGTCACTAACATAAGGTTAATGCA62230.14811295808686517No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG61170.14559006341271963No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC59730.14216273479878605No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA58980.14037766781236233No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC58580.13942563208626965No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA56120.13357061237079984No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA55340.13171414270491916No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC54900.13066690340621723No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT51130.12169396668779392No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA50820.12095613900007213No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA50600.12043251935072116No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA50440.12005170506028408No Hit
TACCCTGTAGATCCGATAAACAGCGGAACCGGCAT49360.11748120859983392No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA49250.11721939877515843No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC49010.11664817733950283No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC48590.11564853982710555No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC48200.1147203049941652No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA48180.11467270320786058No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT46960.11176899424327796No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG46840.11148338352545017No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA46650.11103116655555617No Hit
GTCCAGTTTTCCCATTCCGAAAATAAGGTTAATGC45990.10946030760750328No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA45670.10869867902662915No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT44440.10577116916889423No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT43990.10470012897703998No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43440.10339107985366258No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC43140.1026770530590931No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG42450.10103479143158327No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA42390.10089198607266936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)