FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742434
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2390366.387180107919072No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA1172503.1329877828172785No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA876362.341684582814286No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT752892.0117656049512163No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT646381.7271647275543134No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT549531.4683759286068905No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN491881.314331795831269No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA412821.1030789053327326No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA373490.9979868716455654No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN355500.9499165516345779No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA330690.8836227973559454No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA297250.7942691841726534No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA261900.6998119405712966No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA242420.6477602544226565No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC224590.6001174636613498No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN212170.5669305056548759No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA203930.5449127492963135No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT200390.5354536646471254No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA195920.5235095662341674No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA188790.5044577940452657No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT167760.4482644182903426No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG161370.43118996888121475No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC157110.4198070026084628No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC156340.4177495180943739No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC152540.4075956984144543No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA146740.39209776311352446No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT132430.3538606158451959No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT117820.3148218512337158No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC116870.3122833963137359No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA116850.31222995515752583No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT114420.3057368546779983No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC99780.26661792833220305No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT93720.2504252580005419No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA93600.25010461106328125No Hit
CAACGGAATCCCAAATCGTCGAGCGGAACCGGCAT87990.23511436674634742No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83410.2228763419742339No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA82300.2199103578045732No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC81620.21809335849342967No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80200.21429903640251238No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC77480.2070310391579384No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG74460.19896142457021285No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC73620.19671689600938855No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA73560.19655657254075823No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA70640.1887541637340832No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA69590.18594850303305283No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA66900.178760667522794No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT65750.17568780104071308No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA65430.17483274254135145No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA62960.16823275974940372No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA62390.1667096867974158No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA60180.16080443903619943No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT58140.15535344110276894No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG57090.15254778040173855No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC56630.1513186338089062No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC54320.1451461802666393No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT53780.14370326904896652No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGCG53090.141859549159718No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC51490.13758425666290974No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA50190.1341105815092531No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA50020.1336563316814672No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGT48360.12922071571602867No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG48210.1288199070444529No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC46750.12491870264111538No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC45850.12251385061166076No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT44660.11933410181715963No Hit
ATCACATTGCCAGGGATTACCGCCTTGGCCGTACA43750.11690252920959995No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA41030.10963453196502597No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC39650.10594709218652888No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA39610.10584020987410866No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT39550.10567988640547835No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT39480.105492842358743No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC38460.10276734339202775No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA38270.10225965240803177No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC37970.10145803506488023No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC37570.10038921194067817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)