FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858913
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3061327.933114843480535No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1445733.746469536887719No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT866632.2457878682416528No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN618771.6034826387638177No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA579931.5028325334103154No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570401.4781364596714153No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA511441.3253473193098677No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT488811.2667038619424693No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA444421.1516714681051374No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT406901.054442015147789No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC378370.980509278130914No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA374200.9697031262430638No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA347980.9017565309194585No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT309650.8024280412644701No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN280570.7270700324158642No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA230680.5977849202612238No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA220640.5717672308238098No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN220330.5709638957913795No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT219590.569046257326869No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA204090.5288795057053631No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC179600.46541603814338395No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA172870.4479758937296591No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC161830.41936680096182527No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA158060.40959721040614283No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA156410.40532139491095026No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAC156060.4044144037453034No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA147330.38179145267073916No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT146660.3800552124393579No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT144730.37505380401164784No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA140920.3651805573227486No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA133990.3472221322429399No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA133530.3460300867109468No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT132140.3424280360816634No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA131870.34172835718245004No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA130400.3379189942867331No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC127270.3298079018625193No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT123060.31889809384145223No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA112440.2913773904723947No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC95860.24841192325403555No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA95270.24688299528908786No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA92100.23866824673165732No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA91010.23584361710149984No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCACA85910.2226274601163592No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA80950.20977409959747734No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC80250.20796011726618352No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC79620.2063275331680191No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAA78100.20238860010578108No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA76740.19886429157641028No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA76540.1983460109103263No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC75530.19572869354660236No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC73860.19140104998480142No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC73550.1905977149523713No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC73110.18945749748698662No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT72420.187669429188997No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA72420.187669429188997No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT70380.18238296639494075No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT69300.17958425079808743No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA67500.17491972480333193No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA67070.17380542137125143No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC66850.1732353126385591No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA66480.1722764934063038No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65030.16851895857719518No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT64310.16665314817929297No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA64230.1664458359128594No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA62640.16232550461749204No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC59850.15509548932562098No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT58450.15146752466303334No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC58170.1507419317305158No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA56800.14719170916784077No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT56340.1459996636358477No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC55830.14467804793733366No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA55790.14457439180411688No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC55080.14273449543951883No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA52990.1373184624789416No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC51680.13392372411609177No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA51510.13348318554992042No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA51290.13291307681722805No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTG50880.13185060145175598No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG50720.1314359769188888No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC50430.1306844699530671No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC50370.1305289857532419No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC49110.12726381755691304No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC49050.12710833335708788No Hit
TCCCTGGTGGTCTAGTGGTTAGGCGCCTTGGCCGT47300.12257337752885333No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA46080.11941186546574126No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA45430.11772745330096844No Hit
GTCCAGTTTTCCCATTCCGAAAATAAGGTTAATGC45430.11772745330096844No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT44890.11632809550254178No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA41290.10699904351303074No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA41010.10627345058051323No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCATG40610.10523688924834532No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40070.10383753144991867No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG39940.1035006490169641No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39290.10181623685219128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)