FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4224879
Sequences flagged as poor quality0
Sequence length35
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1022522.420234993712246No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT675681.5992884056561147No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN537021.2710896572422548No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN507551.2013361802787725No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN438111.0369764435857216No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC432871.0245737215195985No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN366100.8665336924442096No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA354000.8378938189709102No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA329440.7797619766151883No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT277040.6557347559539575No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA257390.6092245482059959No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA223720.5295299581360792No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA174230.41239050869859234No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC172170.4075146294130554No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA154780.3663536872890324No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT142990.3384475626402555No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA136100.3221394032823188No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT106330.2516758468112341No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA101750.24083529966183645No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA100830.2386577225051889No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAT99970.23662216125006183No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA97160.22997108319551873No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA96280.2278881833065515No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC96080.22741479696814984No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC91160.2157694930434694No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC90140.213355222717621No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA77330.1830348277429957No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA74660.17671512012533375No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA68020.16099869369039918No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA65800.15574410533414093No Hit
GGTCGCATAGCTCAGTTGGGAGAGCACCCGCCTTG63740.150868226048604No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA60870.1440751320925404No Hit
CGGGAGGATAGGAACTTGCCAAGCCGCCTTGGCCG59450.14071408908988872No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT59410.14061941182220838No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG58520.13851284261632107No Hit
GCGTCATTAGCTCAGCTGGTAGAGCACACGCCTTG56150.1329032145062616No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC54220.12833503634068572No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC53890.12755394888232302No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA53830.12741193298080253No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA49470.11709211080364668No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA49450.11704477216980652No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAAGC47530.11250026332115072No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG44870.10620422502040885No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT44630.1056361614143269No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA44320.10490241258980434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)