FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847791
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1241933.227644121003453No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1119332.909019746654639No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA819732.130391177691304No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN770332.0020058262000195No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA699321.8174583806656857No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT592461.53974059401875No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA475801.236553648573948No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN433411.1263865423044026No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA410611.0671317646930407No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA317600.8254086565512525No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA290080.7538871004168366No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT281280.7310168353738548No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN258110.6708004670731856No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA244930.6365470473838106No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN235430.6118575567124098No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC216560.5628164315577431No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA203340.5284590561181728No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA196130.509721032145457No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT195080.5069921937028284No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT193890.5038995101345162No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA187750.4879423024795266No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA183880.4778845836481243No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC173040.44971257534517856No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG161590.41995524185175337No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT161260.41909760691264153No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA146870.38169952577985655No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA142890.37135592863541705No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA131350.3413646947040523No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA127100.330319396245794No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA126580.3289679714932542No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT102320.26591880899976117No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA94310.24510167002313796No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG92100.23935811482484365No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT89820.23343263706370748No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA87520.2274551814274736No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA83010.2157341705929454No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG81190.21100418395905599No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT79370.20627419732516658No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA76380.19850350499806252No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA75720.19678823511983887No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT75710.19676224618229002No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA71920.18691243885127856No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA71920.18691243885127856No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA71270.18522315791060376No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA68780.17875191246094188No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA67550.1755552731424342No Hit
CTGACCTATGAATTGACAGCCACGCCTTCCGGCAT65280.16965578431884684No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT65120.16923996131806535No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA61820.16066361192694717No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61020.15858449692303975No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC57280.14886463427977248No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG56730.1474352427145861No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG56080.14574596177391133No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA55820.1450702493976414No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT53840.1399244397629705No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT52700.1369617008824024No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCATAATG51910.1349085748160438No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA51910.1349085748160438No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC51360.13347918325085745No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG51130.13288143768723407No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA49720.12921699749284718No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT47230.12274575204318529No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT47040.12225196222975729No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC45710.11879543353576116No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC45710.11879543353576116No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC44910.11671631853185374No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC44560.11580670571764422No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC43880.11403945796432291No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC43500.11305187833746688No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA42900.1114925420845363No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT42890.11146655314698746No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA42220.10972529433121497No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT42000.10915353770514043No Hit
CTGACCTATGAATTGACAGCCACGCCTTAATTACG41830.1087117257668101No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41690.1083478806411263No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA40690.105748986886242No Hit
CATTATTACTTTTGGTACGCGCGCCTTGCGGCATG40560.10541113069810705No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG39570.10283822588077159No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39380.10234443606734356No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC39330.10221449137959936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)