FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258683
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC602702.668369133694281No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA514582.2782302784410207No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN469472.0785121241006377No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT422021.868433950226747No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT400421.7728030006866833No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT289861.2833142145223566No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC264791.1723203300330325No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT239131.0587143038664566No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230971.0225870562624326No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG207570.9189868609273634No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA206510.9142938606258602No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA196610.870463008753331No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166160.7356499340544911No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA148730.6584810706061895No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131900.5839686224228898No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA121270.5369057986446084No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC97440.4314018390362879No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA96770.42843550865703595No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA95900.42458370652278343No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA91890.4068299978350216No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA80590.35680084367748816No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT79380.35144373955973457No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT72020.31885837897571284No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA70070.31022502936445706No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61520.2723711118381818No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGAGAA60840.26936050787117977No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC60790.2691391399324296No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG58340.25829211093367244No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA55300.24483294025766345No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC53660.23757207186665857No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA52380.2319050526346548No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG50900.2253525616476504No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA50740.22464418424364996No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA50080.22172212745214798No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA46540.20604927738863751No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA42800.1894909555701265No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA42780.1894024083946264No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA40750.18041487008137044No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA40660.18001640779162018No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA40610.17979503985287001No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA39420.17452648291061648No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT37450.16580458612386068No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT37420.1656717653606106No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC33790.14960045300734987No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA33460.14813942461159887No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA33280.14734250003209834No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT32900.14566010369759722No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32880.14557155652209716No Hit
TCGAGGAGCTCACAGTCTAGTCGCCTTGGCCGTAC32820.145305914995597No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGACTT32140.142295311028595No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT30080.1331749519520889No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC29870.13224520660933828No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT29310.12976588569533662No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC29100.12883614035258598No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA28670.12693237607933472No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA28100.12440878157758305No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC27670.12250501730433176No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT27420.12139817761058103No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA26310.11648380937032776No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT25990.1150670545623268No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA25810.11427012998282628No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA25290.11196790341982475No Hit
TAGCAGCACATCATGGTTTACCGCCTTGGCCGTAC24680.10926721456707295No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC24260.10740772388157169No Hit
TGTAAACATCCCCGACTGGAAGCTCGCCTTGGCCG24080.10661079930207117No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT23710.10497267655532007No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT23190.10267044999231853No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT23180.10262617640456852No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC22890.10134224235981765No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT22890.10134224235981765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)