FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874280
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC744121.920666549655678No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT591731.5273289488627564No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC558751.4422034545773665No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT552871.4270264410419484No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT430641.1115355627368182No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT388031.0015538371000547No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA379400.9792787304996026No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN338580.8739172181669884No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA321950.8309931135591645No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA267270.689857212178779No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA263550.6802554281053512No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC257760.6653107157975159No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA215480.5561807613285565No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA190020.49046532517009617No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA172670.44568281074160876No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170400.439823657557017No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151410.39080809853701853No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC149230.3851812465800097No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT146150.3772313823471716No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA137010.35363990212374946No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCCCGTA124180.3205240715694271No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCTTACG116650.3010882021949885No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT109640.28299451769102907No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG107370.27713536450643733No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT106760.27556087840837523No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA106260.2742703160329145No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA101210.2612356360407611No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC101170.2611323910507243No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA97870.25261467937268345No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA96970.25029166709685413No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA94900.2449487388624467No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT94760.2445873813973177No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG88740.2290490103967705No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA88250.22778425926881898No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA86730.22386094964741837No Hit
CTGACCTATGAATTGACAGCTGTATTCCATAATGC84790.2188535676306307No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC82490.21291698070351134No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG79990.2064641688262077No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA78810.20341844162012038No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA76190.19665589477270615No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC72570.18731222317437046No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA71930.18566030333378072No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC71800.18532475711616095No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT69190.17858802151625594No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68400.17654893296302798No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67880.17520674809254883No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCTAC66710.17218683213397068No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT66480.17159317344125877No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC66250.17099951474854683No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA62070.1602104132896951No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC61800.15951350960694632No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC61690.15922958588434497No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT59370.15324137646220717No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC57950.1495761793158987No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC57700.14893089812816832No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG56590.1460658496546455No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC56330.14539475721940592No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56250.1451882672393322No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGAATGC56180.1450075885067677No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC56140.14490434351673084No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA55930.14436230731903735No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA55150.1423490300133186No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC54100.13963884902485107No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT53990.13935492530224972No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC53330.13765138296664153No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAA53000.13679961179883746No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA52830.13636082059118082No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT52560.13566391690843202No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA51840.13380550708776856No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT51810.13372807334524092No Hit
AGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51480.13287630217743684No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC50070.12923691627863756No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA49400.12750756269552022No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC49110.12675903651775297No Hit
TGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48990.1264493015476424No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA47840.12348100808408272No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT47720.12317127311397215No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG46330.11958350971019133No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCGACTCTCA46180.11919634099755308No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC46100.11898985101747937No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG45590.11767347739450942No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45290.11689913996923298No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA45020.11620223628648421No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA45010.11617642503897498No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC44380.11455031644589447No Hit
CAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43690.11276934036775865No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC43600.11253703914017572No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC43350.11189175795244535No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG42910.1107560630620399No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG42580.10990429189423584No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGATGCA41710.10765871336093416No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC41600.10737478963833279No Hit
CGCCTTGGCCGTACAGCAGCGCGAACCGGCATGTC41070.10600679352034442No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA40990.10580030354027072No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC40340.10412257245217175No Hit
TCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39950.1031159337993124No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT39380.10164469269128716No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA39370.10161888144377795No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA39340.10154144770125029No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA39330.10151563645374108No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAG38870.10032831906831721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)