FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169536
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT633741.9994724779904691No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC545241.7202517971084728No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA327291.0326117135126405No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA322241.0166787820046848No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA277690.8761219307810354No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCGACTCTCA264510.834538557063242No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT230430.7270149321541071No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA223520.7052136337937162No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA206050.6500951558840159No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC188900.5959862894758097No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC161660.5100431104111138No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA159890.5044586967934739No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT125740.396714219368387No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC124400.39248647120588No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC98880.3119699539617155No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG90520.2855938534851789No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG90250.2847419937807931No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC89590.2826596700589613No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA88390.278873626928358No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC84240.26578022776835475No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT84100.26533852273645103No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG80180.25297078184314675No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA77130.24334792221952992No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76810.2423383107180357No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT74650.2355234330829497No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC73350.2314218863581294No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA72560.22892940796381553No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG71970.22706793675793552No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT71160.2245123576447783No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT69240.21845468863581297No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA67350.21249167070511266No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC64650.20397307366125514No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG64270.20277416000323076No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA63480.2002816816089169No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT63000.19876726435667558No Hit
CTGACCTATGAATTGACAGCTTATTCCATAGCATG62790.19810470680881997No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC62250.19640098740004844No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA60980.19239409175349326No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC59060.1863364227445279No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA58860.18570541555609402No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC58520.1846327033357564No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT57310.18081510984573138No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA56810.17923759187464663No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55460.1749782933527179No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA54980.17346387610047653No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT52960.16709070349729424No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA52590.16592334019869154No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT52180.16462977546240207No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51400.1621688474275099No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC49660.15667908488813503No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA47850.15096846983280834No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCGACTCTCT47210.1489492468298199No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA46990.1482551389225426No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC45930.14491080082384297No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA45030.14207126847589047No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT44620.140777703739601No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC44600.1407146030207576No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA44560.14058840158307084No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA44360.13995739439463695No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC40890.12900941967530893No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC40400.1274634520636459No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC39990.12616988732735643No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA39580.12487632259106694No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG39450.12446616791858492No Hit
ATCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGC39270.12389826144899442No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG39100.12336190533882561No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37000.11673632986026977No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC36630.11556896656166706No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG36280.11446470398190776No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC35530.11209842702528067No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG34790.10976370042807526No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34470.10875408892658105No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT34380.1084701356917858No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG34370.10843858533236411No Hit
CTGACCTATGAATTGACAGCTGTATTCACGAATGC33740.10645091268879736No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA33620.10607230837573701No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA32470.10244401704224215No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA32160.1014659559001696No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG32120.10133975446248283No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC32110.10130820410306114No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA32060.10115045230595268No Hit
AGCAGCATTGTACAGGGCTCGTACGCCTTGCGATG32050.10111890194653098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)