FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484569
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT40765611.698893033830009No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1970225.65412824369384No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC901262.586431779654815No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC568961.6327987765488359No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN543571.559934671978084No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN494301.4185398538528007No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA474211.360885664769445No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA456701.3106355477535385No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT311200.8930803206938935No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA292900.8405630653317526No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA275000.7891937281196039No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT259910.74588851591115No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN245690.705080025678929No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA242300.6953514193577456No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229440.6584458508354979No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA219080.6287147707507013No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC202570.5813344491097752No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA196930.5651488031948858No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG181510.5208965585126883No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT164140.4710482128492792No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA152710.4382464517132535No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT137460.39448207224480275No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA134290.3853848209061149No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC133990.38452388229362083No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA130030.3731594926086985No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG129970.3729873048861997No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA129050.3703470931412177No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA124000.35585462649756683No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA113140.3246886487252799No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA113090.3245451589565309No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA109480.31418519765285174No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA108300.3107988391103749No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA103040.29570371543797813No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA98330.28218697922182057No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA95900.2752133764606182No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA93140.26729274122567237No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG88690.2545221518070097No Hit
GTCCAGTTTTCCCAGGAATCGGAGCGGAACCGGCA78110.2241597167397173No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC76730.22019939912224437No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC75220.21586600810602402No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC74520.21385715134353778No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA73250.21021251121731266No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC72770.20883500943732208No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC71600.20547734884859506No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA67140.19267806147618255No Hit
GTCCAGTTTTCCCAGGAATAAAATAAGGTTAATGC65210.18713935640247042No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG64800.18596274029872847No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC61060.17522970559630185No Hit
TGAGATGAAGCACTGTAGCTCGCCTTGGCCGTACA57670.16550109927511839No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT57290.16441057703262585No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA55280.15864228832891528No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT54930.15763785994767215No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA52110.14954503699022748No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC51830.14874149428523298No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA49400.14176789152403066No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA48620.13952945113154597No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA47160.13533954988407462No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGATCT47100.1351673621615758No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC45340.13011652230161033No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGAGAG44950.128997302105368No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC44660.12816506144662365No Hit
GTCCAGTTTCTTTGAAGGCGGGGCGGAACCGGCAT44090.12652927808288486No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA43650.1252665681178935No Hit
GTCCAGTTTTCCCAAAGGCGGGGCGGAACCGGCAT43220.12403255610665193No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT41970.12044531188792645No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA41920.12030182211917745No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC41720.11972786304418136No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG41580.11932609169168411No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA41490.11906781010793588No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT41350.11866603875543862No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC40770.11700155743795No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA40250.1155092638429602No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC40090.11505009658296335No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA39000.11192201962423474No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT38680.11100368510424102No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA38650.1109175912429916No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGATAG37960.10893743243425515No Hit
TCCCTGAGACCCTTTAACCTGCGCCTTGGCCGTAC37800.10847826517425826No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA37730.10827737949800965No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA37270.10695727362551868No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA37120.10652680431927161No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)