FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246696
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT2333815.4955899833658926No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1694713.990655323573903No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA765301.8021068614282727No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN735151.7311104915444853No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN477961.12548673133184No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA399440.9405900492995025No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT394360.9286278085363303No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA383860.9039027045967029No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT383480.9030078913112688No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN378210.8905982439053797No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA370980.8735732437640933No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA348950.8216976209269512No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA334030.7865644256146425No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT328280.7730244877429417No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC316030.7441785331467098No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN254710.5997839261392857No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT236300.5564325772318056No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA219890.5177907719318736No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA197500.4650674312453729No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC194900.45894502455556035No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA194690.4584505224767678No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA179440.42254025246921373No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT178000.41914938107177907No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC164920.38834896587841466No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT159170.3748090280067139No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA151060.35571182867810647No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC149150.35121421453289803No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA137710.324275625097723No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC134580.3169051893519103No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA133990.3155158739876836No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC125960.29660705640337803No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA123890.29173267876956577No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC123680.29123817669077323No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA121610.28636379905696097No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC119230.2807594421639788No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA116260.2737657699067699No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT112470.26484118477046625No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAA109950.25890715982495566No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA105280.2479103755013309No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT102690.24181151652955618No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA95440.22473942095219437No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACC90720.21362489803838092No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG89600.210987553618154No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC89220.21009274033271982No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACCTA88490.20837375691596477No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA87860.20689025067958713No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC85510.20135653694071817No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA83440.1964821593069059No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA81450.19179616341739555No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA80440.18941784389558378No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACCTA79130.18633309283263977No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC77050.18143516748078978No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG73630.17338184791188255No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA72360.17039128772108952No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA71770.16900197235686285No Hit
TAGCACCATCTGAACGATTGGCATAAGGTTAATGC70530.16608205532018305No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA70330.16561110095942821No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC69800.164363071903428No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC65010.15308371496335033No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG64750.15247147429436908No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT64190.15115280208425563No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC62680.14759709666055681No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA61330.14441815472546188No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA59920.14109792648214048No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA59660.1404856858131592No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG58990.1389079887046306No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA58500.13775415052078133No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG57010.13424554053315801No Hit
AACCGGGCGGAAACACCACGCCTTGGCCGTACAGC55800.13139626665059143No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA54890.12925342430915704No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC54850.12915923343700608No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAC54680.1287589222303645No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC54180.1275815363284775No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT51870.12214201346175944No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA51810.122000727153533No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA51290.1207762458155705No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC48370.1139003121485503No Hit
TATTGCACTTGTCCCGGCCTGTCGCCAACCGGCAT47310.11140425403654983No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA47230.11121587229224791No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC47200.11114522913813468No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC46460.10940269800334189No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA46320.10907302995081354No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA45980.10827240753753037No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA45210.10645923324862434No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC43810.10316255272334068No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA43050.10137292615247241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)