FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205503
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2150245.112919905181378No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1532043.6429411654206407No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1041532.476588412848594No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN748511.7798346594925745No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA712711.6947081003152298No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA592721.409391456860214No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN563821.3406719719377207No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT533451.2684570668478894No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA461831.0981563917562298No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA449761.0694559009944826No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA411820.9792407709612858No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT410540.9761971397951683No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC399440.9498031507764946No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT359190.8540952176231952No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA348710.8291754874506094No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN295780.70331658305796No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN259460.6169535487193802No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA232940.5538933154963865No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT225770.5368442252924324No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT223150.5306142927492858No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT171480.4077514627857832No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA163930.38979879457938804No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA162950.38746851446782943No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT162100.38544735314657963No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC156270.37158456431965453No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT139740.33227892121346725No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC119780.2848172977168248No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA118780.28243946086829563No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA117000.27820691127791375No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA112600.26774442914438534No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC109600.2606109185987978No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT107420.2554272342690042No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA106270.25269272189319564No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGACCA103930.24712858366763737No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC97870.23271889236555057No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC97160.23103062820309483No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT94350.22434890665872786No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT93970.22344532865628675No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC90570.21536068337128758No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG89630.21312551673367017No Hit
CGCCTTGGCCGTACAGCAGATACCGACCCAGAAAC83020.19740801516489231No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA78760.187278430190158No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA77810.18501948518405525No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC76530.18197585401793792No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA75870.18040648169790868No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA75290.17902733632576176No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC73840.17557947289539444No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA72670.1727974037826153No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG71600.17025311835468906No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA69530.16533099607823368No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA65000.1545593951543965No Hit
CCACTACGCCTTGGCCGTACAGCAGATACCGACCC62620.14890014345489708No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC61360.14590406902575032No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT60760.1444773669166328No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA60750.14445358854814752No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT60650.1442158048632946No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG59820.14224220027901538No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA59160.1406728279589861No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA58190.13836632621591283No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC57630.13703473758073648No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA57500.1367256187904277No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC56600.13458556562675142No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG56070.13332531209703097No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA54730.13013901072000186No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA53570.12738071997570802No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA51700.12293416506895846No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA49170.11691823784217964No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT48740.11589576799731209No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA48520.11537264389063567No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG48430.11515863857426804No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT47630.11325636909544472No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA44930.10683620960441594No Hit
GCTAAACCTAGCCCCAAACCCACACGCCTTGGCCG44460.10571862628560721No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC44090.10483882665165142No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC43270.10288900043585748No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA43150.102603660014034No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC42800.10177141711704878No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA42070.10003559621762248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)