FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555568
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1848715.199478676824631No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1613744.538627864802473No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT1016762.8596274913037805No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA962212.7062061532784636No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA866952.4382883409908067No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN632471.778815649145228No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA512291.4408105821629624No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA423041.189795835714575No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA420241.1819208632769787No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN404061.136414772548296No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA370051.0407619823330618No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC289880.8152846465037371No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN289390.8139065263271579No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA251770.7081006466477368No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN247540.6962038132866535No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA245460.6903538337615818No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA208370.5860385738649915No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA174330.4903014089450687No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA163960.4611358860243989No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC154500.43452972914594795No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG152930.4301141196005814No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC151800.42693600572397994No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG142520.4008360970736602No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC123770.34810190664332674No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA116530.32773947791182734No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA112860.31741763903826337No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA111560.3137614018350936No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG101780.2862552481066316No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA100600.282936509722216No Hit
TAGCTTATCAGACTGATGTTCTGCGGAACCGGCAT97320.27371154200960296No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA87460.24598038906863828No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA86620.24361789733735933No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT86420.24305539930610243No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA85550.24060853287013495No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC84400.23737416919040782No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA81010.22783982756060356No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG78920.221961723133969No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG78120.21971173100894148No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC77880.2190367333714332No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA77650.2183898606354878No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA75910.2134961277635528No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT75560.21251175620885326No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT73410.20646490237284168No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC69350.19504619233832682No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA66090.18587747442883948No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC65710.1848087281694514No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT62810.1766525067162265No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC62560.17594938417715536No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA62540.1758931343740297No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT61490.17294001970993103No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC57900.16284318004886983No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT57660.16216818241136158No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT57360.16132443536447622No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT56790.15972131597539407No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCTACG54950.1545463340878307No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC50810.14290262484081306No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC45650.1283901756343853No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA44650.12557768547810083No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT44310.1246214388249641No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA44260.1244808143171499No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA43910.12349644276245034No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC43710.12293394473119343No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA42880.12059957790147735No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT40110.11280898016856941No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT39500.1110933611732359No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG38590.10853399513101704No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA37740.10614337849817526No Hit
TCCCTGAGACCCTAACTTGTGCGCCTTGGCCGTAC37630.10583400458098396No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA37490.10544025595910414No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA37250.10476525832159586No Hit
GTCCAGTTTTCCCAGGAATCCCTATAAGGTTAATG36460.10254339109813115No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA36390.10234651678719124No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG36010.10127777052780315No Hit
TACCCTGTAGATCCGAATTTGTGCGCAGGTTAATG35920.10102464641373754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)