FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533042
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA2167384.781292562477912No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1855354.092946855555276No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1062652.3442315337029744No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA851431.8782751185627666No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN675311.4897501501199415No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA668261.474197680056792No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC520431.1480811340375845No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA499001.1008060370938544No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT494311.0904597839596457No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA462391.0200434939715979No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT436870.9637457583671186No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA429690.9479065051680527No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT413470.9121247939021964No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN399700.8817478417362998No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC267780.5907291395049946No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC257880.5688895006929121No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA256970.5668820187414986No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT237250.5233792230471281No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA232780.5135182952198546No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN220890.4872886684041313No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC216670.47797924660746577No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA211640.46688294527162993No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA210790.46500782476756225No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT205640.4536468005370345No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT204830.4518599209978641No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA203900.44980831856400183No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT202570.44687430648116655No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC178040.3927605347578955No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN173110.3818848358343029No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA162740.3590083656846771No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA155400.342816148626022No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA151510.3342347147897593No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA149570.3299550279922401No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA140880.310784678368301No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT125150.27608391892243667No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA125040.27584125626896905No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT124900.2755324128918285No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC98370.21700659292369232No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT95250.21012379766170267No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA92870.20487346025031317No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT84940.18737968895942284No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA81260.17926152018887095No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC80380.1773202189611303No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA79960.1763936888297086No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC79920.1763054478648113No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA78180.17246696589177862No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT78030.17213606227341374No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA77910.17187133937872184No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT77340.17061390562893528No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC76670.16913586946690545No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC76600.16898144777833518No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA73920.16306930313021586No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC73620.16240749589348608No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC71770.15832635126698583No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT71590.15792926692494796No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC70970.15656153196903977No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC70710.1559879656972073No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC70650.15585560424986136No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA69900.15420108615803693No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA68810.15179651986458542No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC66710.147163869207477No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAC66550.1468109053478878No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65140.14370041133525785No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA64350.14195765227853613No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA63940.14105318238833878No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC63700.14052373659895495No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA63530.14014871249814143No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA63130.1392663028491684No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA62020.13681761607326823No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC61610.13591314618307088No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT61110.1348101341218546No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA59790.13189818228024358No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC59770.13185406179779494No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC57200.12618457980314324No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA55590.12263288096602679No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC55500.12243433879500787No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA53590.11822083272116164No Hit
TCTCCCAACCCTTGTACCAGCGCCTTGGCCGTACA53220.11740460379586158No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC53030.1169854592125994No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT50960.11241898927916397No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC50550.11151451938896662No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT47380.10452142292085537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)