FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292385
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT470452.0522294466243673No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA357761.5606453540744682No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN344601.5032378941582676No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT291701.27247386455591No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA246351.074644965832528No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA235751.028404914532245No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC217050.94683048440816No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA203230.8865439269581681No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA173400.7564174429687858No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT161470.7043755739110141No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN156740.6837420415855103No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA134490.5866815565448212No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTCG130550.5694942167218857No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA128140.5589811484545572No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC128080.5587194123151216No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA127040.5541826525649051No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126870.5534410668365043No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT125370.5468976633506152No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119900.5230360519720727No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC107750.47003448373637063No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC103510.4515384632162573No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA96020.41886506847671745No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA90570.39509070247798694No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA81700.35639737653142906No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG79510.3468440074420309No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC65630.2862957138526033No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC65000.28354748438852984No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC63070.2751283052366858No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTCA62160.2711586404552464No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG61530.26841041099117297No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC61400.26784331602239586No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGAGAT61380.26775607064258405No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA55150.2405791348311911No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA55090.24031739869175553No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC54580.2380926415065532No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA52610.22949897159508545No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA51150.22313005886882004No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA51140.2230864361789141No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT50080.21846243104888577No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA48310.21074121493553657No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA43980.1918525902062699No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT43880.19141636330721062No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA43060.18783930273492455No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA42970.1874466985257712No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA41280.18007446393166943No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA41280.18007446393166943No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG40640.17728261177769006No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG39680.173094833546721No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC37840.1650682586040303No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA37680.16437029556553545No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTTA35760.15599473910359735No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA35340.15416258612754838No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC35060.15294115081018242No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG34770.1516760928029105No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC34770.1516760928029105No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC34740.15154522473319273No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC32490.141730119504359No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC31920.13924362617972114No Hit
AACCCGTAGATCCGATGAACGTATATCCAATTACG31580.13776045472291956No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA31210.13614641519640025No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG30530.13318007228279716No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA30130.13143516468656005No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT27470.11983152917158331No Hit
AACCCGTAGATCCGATCTTGCATATAGGTTAATGC27090.11817386695515805No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTAC26740.11664707280845059No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA26390.11512027866174311No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC25340.11053989622162072No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA24860.10844600710613618No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA24460.10670109950989907No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA24000.1046944557742264No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG23800.10382200197610784No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC23750.1036038885265782No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC23270.10150999941109368No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT23210.10124826327165813No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)