FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400693
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1184802.6923032349677656No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1174352.66855697500371No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1079282.452522818565167No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA632631.4375690374220607No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT554871.260869594856992No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN531361.207446190861303No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA504331.1460240466671954No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT352430.8008511386729318No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN337020.7658339266111042No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA315450.7168189192020439No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA296570.6739165854105251No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA264830.6017915814622833No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGCA260540.5920431168454605No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN239280.5437325439425109No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC228650.5195772574910361No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT218370.49621730032065403No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217420.4940585494148308No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA215840.49046820580304057No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC214090.4864915593975767No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT213290.4846736638979361No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC204520.46474498448312573No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA193410.4394989607318665No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA190850.4336816951330165No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATAC189630.4309094044960646No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA186640.42411502006615776No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGAC180940.4111625146312183No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA169910.3860982804299232No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC165430.3759180656319357No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA158970.36123855947233763No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC156130.3547850304486134No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG152700.34699080349390427No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA142570.32397170172970485No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA141400.3213130295614804No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA125100.2842734087563027No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG121350.2757520236017373No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC105840.24050757460245464No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC105510.2397576927088529No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA105210.23907598189648765No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGAA102160.23214525530410776No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT97980.2226467513184855No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC96880.22014714500647967No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC96550.2193972631128779No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT96430.21912457878793182No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA96080.21832924950683902No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC95440.21687493310712652No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATCA94500.21473890589504882No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT94020.21364816859526445No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG87440.19869597811072026No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC81930.1861752228569455No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT81580.18537989357585272No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT81310.184766353844724No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG80780.1835619980762121No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA78070.17740387707117947No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGCC77750.17667671887132322No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC75250.17099579543494628No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG74660.1696550975039613No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT70540.1602929356808121No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG70200.1595203300934648No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG67540.15347582755715974No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67010.15227147178864783No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA64710.14704502222718105No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC63950.14531802150252246No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA63850.14509078456506735No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC60810.138182781666433No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT60500.13747834716032226No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC60290.13700114959166657No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA59460.13511508301078942No Hit
TCCCTGGTGGTCTAGTGGTTAGGACGCCTTGGCCG59450.13509235931704394No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC59130.1343652011171877No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC58780.1335698718360949No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG58290.13245641084256501No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG58130.1320928317426369No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC56350.1280480142559365No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC56340.128025290562191No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA55320.1257074738001492No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA55320.1257074738001492No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT54270.12332148595687088No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC52010.11818593117038613No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA51350.1166861673831826No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG51190.11632258828325448No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT49280.1119823627778625No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA49240.11189146800288044No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT48870.11105069133429667No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA48080.10925551952840155No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATCC47510.10796026898490761No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA47140.10711949231632381No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT46910.10659684736017713No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC46630.10596058393530293No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46310.10523342573544668No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA45300.10293833266715037No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA45170.10264292464845878No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATAA44600.10134767410496483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)