FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864786
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1511213.910203566251792No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA778242.0136690621421214No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT696901.803204627630094No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA693541.794510743932523No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT636261.6463007266120298No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN597281.5454413258586632No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA568071.469861461928293No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA542421.40349297477273No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA338750.8765038995690835No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT338030.8746409244910327No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC312180.8077549442582331No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN289800.7498474689154846No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA271560.7026521002715286No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC264730.6849797116839069No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA253190.655120361127369No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA238980.6183524779897257No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACCA222830.5765649120028897No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA195520.5059012323062648No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN193080.49958781676398123No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA173470.4488476205409563No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC168050.43482355814785084No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT166530.43089061076085455No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC145220.37575172338132046No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA130870.33862159508961165No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC125080.3236401705036191No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT117260.30340619118367745No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT115030.29763614337249206No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC107990.2794203870537722No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA104570.27057125543303046No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGACTG94980.24575746237954701No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93830.24278187718543795No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA93060.24078952883807794No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA93030.24071190487649252No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA88360.22862844152302353No Hit
CGCCTTGGCCGTACAGCAGTTCGACGACCAGAGGT84820.21946881405594No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC84320.21817508136284908No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA83930.21716596986223816No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA76370.19760473154270378No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA76200.1971648624270529No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC73700.1906961989615984No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA71320.18453803134248573No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA70720.18298555211077663No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC68270.17664626191463126No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC64490.16686564275486404No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC63320.16383830825303136No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT62590.16194945852111864No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC62560.1618718345595332No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61960.1603193553278241No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA61900.1601641074046532No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT58290.1508233573605369No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA57440.14862401178228238No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT52350.13545381296661704No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG51340.13284047292657342No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA51250.13260760104181707No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT50940.13180548677210072No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA50810.13146911627189706No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG50720.1312362443871407No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA50470.13058937804059526No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC50420.1304600047712862No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG49370.12774316611579528No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC48590.12572494311457347No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA47810.12370672011335168No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT47680.12337034961314805No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA46310.119825522034079No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA45990.11899753311050082No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC44720.11571145207004993No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC43970.11377085303041358No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC43810.11335685856862449No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC43330.11211487518325723No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA41550.10750918679585364No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC40590.1050252200251191No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG39780.10292937306231185No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA39680.10267062652369369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)