FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3368852
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2267126.729651525207994No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1041943.0928636817527155No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT765952.273623180834302No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN624821.8546970896910875No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA505791.5013719807222163No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT474671.408996299035992No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN430721.2785364272458393No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA426421.2657724352390665No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA408061.2112731577403815No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT391511.1621466303654775No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA374771.1124561126460883No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA366731.0885904159636577No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA253810.75340204912534No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230470.6841202878606718No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC214540.6368341500309304No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT213280.6330940035359226No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT212430.6305708888369095No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA196830.584264313184432No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA189790.5633669867361344No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN177930.528162115759315No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC174100.516793257762585No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA164910.48951393531090115No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA164390.48797038278915195No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA154840.4596224470531801No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC133540.39639616106614356No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA126690.3760628249623314No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA126210.37463800724994745No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA125330.37202584144391027No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA115180.3418968835674586No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA114890.34103605619955996No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT108520.3221275378081317No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC99120.29422485760727984No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT91430.2713980905067958No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT88370.2623148775903483No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA87020.2583075777742685No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC85470.25370660391136207No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC82990.2463450457307118No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA77810.23096888791790202No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA77610.2303752138710754No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC76730.22776304806503817No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT75470.22402290157003038No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA74190.22022338767033992No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA73620.218531416636884No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG73270.21749248705493743No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT71270.2115557465866711No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA71050.21090270513516177No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA69100.2051143831786021No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT65790.1952890777036213No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATAC63100.18730416177380307No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC61070.18127837019851273No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA60760.18035817542593144No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT55240.16397277173351635No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA54440.1615980755462098No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC54400.16147934073684447No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT54130.16067788077362852No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA52900.15702678538564474No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC51070.15159466785718104No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47490.14096790241898427No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT46550.13817763439889907No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA45590.13532799897413125No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA45010.133606344238334No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44700.13268614946575275No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA44300.13149880137209946No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC43960.1304895554924942No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC42420.1259182653319291No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG42140.12508712166637181No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA41530.12327641582355058No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC40410.11995184116132143No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT40180.11926911600747078No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT40020.11879417677000947No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG39160.11624137836865496No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA38670.11478687695392971No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA37870.11241218076662317No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC37360.11089831194721525No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC37120.11018590309102329No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT36690.10890950389034604No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA36640.10876108537863938No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC36600.10864235056927404No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG36260.10763310468966877No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT35080.10413042781339163No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA34140.10134015979330645No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT34080.10116205757925846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)