FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991775
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC963823.221565792882152No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA738862.469637589725163No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT570921.908298585287998No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT483151.614927593151223No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA436381.45859899223705No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN360971.206541267307869No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA310931.039282700069357No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA272280.9100951776119528No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA267080.8927141914081106No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265660.8879678451755229No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG255430.8537740973168102No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA214380.7165645812268637No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA161320.5392116720007354No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN156780.5240367340458424No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGAT154450.5162487152275822No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT148050.49485673220746884No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT127380.42576731204719603No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC127350.42566703712678927No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121520.40618027759440467No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA118290.3953840111639411No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA111950.37419257798464123No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC97040.3243559425424706No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC95600.31954274636294505No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT94900.31720299822012016No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA88720.29654636461632305No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA86570.2893599953205037No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA85170.2846804990348539No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA81430.2721795589574751No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTT80010.2674332127248874No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT78160.26124959263313585No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA77850.2602134184555991No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA75080.2509547008047063No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA73060.2442028561639829No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA70060.23417536412330472No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG69430.2320695907947623No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT68980.23056546698866057No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT68780.22989696751928201No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT66750.2231116979050898No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA64360.21512312924601615No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA63470.21214830660728165No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA62160.20776963508285215No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT59040.19734104336054684No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT58590.1958369195544451No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT57170.19109057332185742No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGCG57120.19092344845451278No Hit
ACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGTA57090.190823173534106No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC56080.18744725121374434No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT54200.181163356201586No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT52770.17638358499552942No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA51620.1725397130466028No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC51310.17150353886906602No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC47680.1593702734998454No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA47440.15856807413659116No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC47220.15783272472027474No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT46410.15512530186929163No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA46310.15479105213460237No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA45630.1525181539387153No Hit
TCAATCCCCGGCACCTCCACCACGCCTTGGCCGTA42350.14155476264090716No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT39030.1304576714492233No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTT36590.12230197792280502No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC35840.11979510491263547No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC35070.11722138195552807No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTT35060.11718795698205915No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA34860.1165194575126806No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGA34820.1163857576188049No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTA34620.11571725814942635No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGGC34520.11538300841473707No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA34500.11531615846779922No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG34240.11444710915760711No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA33950.11347778492700822No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT33360.1115057114923415No Hit
CGCCTTGGCCGTACAGCAGAAGTTTGCTGAGAGTC33290.11127173667805901No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC33130.11073693710255618No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG33120.11070351212908724No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC32490.10859773880054482No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC32430.10839718895973127No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA31700.10595716589649956No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTT31340.10475386685161818No Hit
CGCCTTGGCCGTACCTACTCCTGGGTAGTCTCTGA31190.10425249224958427No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC30830.10304919320470289No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA30710.10264809352307576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)