FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4079526
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2684346.580029150445419No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1254933.0761662016616635No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1090162.6722712393547683No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA744301.8244766671422121No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT588861.443452008885346No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA556611.364398707104698No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA466091.1425101837811549No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN446971.0956419937022095No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN412191.0103869910376844No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT373030.9143954469220198No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA351960.8627472897586631No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA313510.7684961439147587No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC286360.7019442945087248No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA284070.6963308972660059No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA263030.644756278057794No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC257260.6306124780182796No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA241280.591441260577822No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234580.5750177839288192No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN225680.5532015239025319No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA216190.5299390174250636No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC202330.4959644821481712No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT188550.4621860480849981No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA183190.4490472667657958No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT158660.38891773210907343No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA155160.38033830400884805No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC129040.3163112577294519No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA119260.2923378843522507No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC118360.29013174569790706No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT113520.2782676222678811No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC111530.27338960457661016No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA105380.25831432377192837No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT102030.250102585447427No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC101990.2500045348405673No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT100440.24620507382475315No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAT99610.24417052373241402No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC93910.23019831225490414No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA92160.22590859820479145No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA91600.22453588970875538No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT90660.222231700447552No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC87720.2150249808433627No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCA86510.2120589499858562No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG86150.21117649452411874No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAC84730.20769569798059873No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA84510.2071564196428703No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT82310.20176363626558577No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA81050.1986750421495046No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA80830.19813576381177617No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC79030.19372348650308885No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT77840.19080648094901223No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA75450.18494795718914403No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT70950.17391726391742568No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA68760.1685489931918561No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC67430.16528881051377048No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCG64490.15808209090958117No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC64170.1572976860547034No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC62730.15376786420775354No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA62170.1523951557117175No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA60520.14835056817875408No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT59680.14629150543470001No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC57950.14205081668801717No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC57680.1413889750917141No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT57240.1403104184162572No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA57020.13977114007852873No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA56890.13945247560623464No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA56530.13857002014449718No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA56170.13768756468275972No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG56080.13746695081732535No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA55850.13690315982788195No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT54660.13398615427380534No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC54500.13359395184636647No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT53990.13234380660890505No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT53020.1299660793925569No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA51450.12611759307331294No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG51000.1250145237461411No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT49510.12136213864061658No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA49240.1207002970443135No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA48330.11846964573825487No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA48260.11829805717625039No Hit
CCACTACGCCTTGGCCGTACAGCAGTATAACCTAT48260.11829805717625039No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC47500.11643509564591573No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC46630.11430249494671686No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA46220.11329747622640474No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC45730.11209635629237319No Hit
ACCACAGGGTAGAACCACGGAACGCCTTGGCCGTA43810.10738992716310669No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40870.10018320755891738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)