FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3829974
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1500373.9174417372023935No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA595811.5556502472340543No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN477581.2469536346721937No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT462511.2076061090754142No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA407731.0645764174900403No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA386751.009797977740841No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN372380.9722781407915562No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT363060.9479437719420549No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA350630.9154892435301129No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA293700.766845936813148No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC264700.6911274071312233No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT238570.622902400904027No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN231480.6043905258886875No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC229140.5982808238384908No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC215830.5635286296982696No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT209430.5468183334926033No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT203990.5326145817177872No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN180180.47044705786514474No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC177020.4621963491135971No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC137440.3588536110166805No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC136340.35598152885633166No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG129850.33903624411027333No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA124450.32493693168674254No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT120270.3140230194774168No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA117850.3077044387246493No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC117460.30668615504961655No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC112520.29378789516586795No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA100440.26224721107767307No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG97890.255589202433228No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA94670.2471818346547522No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC89730.23428357477100367No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAT86480.22579787747906382No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC86000.22454460526363887No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG83300.21749494905187344No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG80370.20984476657021695No Hit
TAGCACCATCTGATTAGCCAATGCGGAACCGGCAT78250.20430948095209No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA68480.1788001694006278No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA68470.17877405956280643No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT68270.17825186280637936No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA66370.17329099362032224No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC65600.17128053610807803No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA62880.1641786602206699No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA62510.16321259622127982No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA59520.1554057547126952No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA58740.1533691873626296No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC57490.15010545763496044No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC56610.14780779190668136No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC54280.141724199694306No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT53530.1397659618577045No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA53400.13942653396602694No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG51760.135144520563325No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA51210.13370847948315054No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA50460.13175024164654903No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC49350.12885204964837882No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT48920.12772932662206063No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC48570.12681548229831324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG47650.12441337721874875No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG47030.12279456727382485No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAA45950.11997470478911867No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA45940.11994859495129731No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC44710.11673708489927087No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA43990.11485717657613342No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC43380.11326447646903086No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG42960.112167863280534No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT42560.1111234697676799No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG41990.10963520901186274No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT41970.10958298933622004No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG41840.10924356144454243No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT41420.10814694825604561No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC41260.10772919085090395No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT39930.10425658242066395No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA39430.10295109052959629No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA38770.10122784123338696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)